中药淫羊藿对大鼠乳腺癌骨转移模型疼痛和破骨细胞的影响

Effects of epimedium brevicornum on cancer pain and osteoclast in rat model of bone cancer pain

目的:研究中药淫羊藿对大鼠乳腺癌骨转移模型的疼痛、肿瘤生长及破骨细胞活性的影响。方法:将雌性SD大鼠随机分为4组:假手术组、模型组、淫羊藿组和唑来膦酸组,每组16只。胫骨内注射MRMT-1细胞建立乳腺癌骨转移模型,各组分别给予对应药物。造模后第19天时观察疼痛行为(机械痛觉超敏、过敏和热痛觉过敏);第21天取材,测定肿瘤体积,核医学方法测定骨矿物质含量和骨密度,抗酒石酸酸性磷酸酶染色计数破骨细胞。结果:与模型组相比,淫羊藿组机械痛觉超敏、过敏和热痛觉过敏减轻(P<0.01,P<0.05),骨矿物质含量和骨密度升高(P<0.05,P<0.01),破骨细胞减少(P<0.05),而肿瘤体积也有下降趋势但差异无统计学意义。结论:中药淫羊藿可以减轻乳腺癌骨转移大鼠的疼痛,具有减轻骨吸收和保护骨质的作用,抑制破骨细胞过度激活是其重要作用机制。

Objective： To study the effect and mechanism of action of Epimedium brevicornum on the rat model of bone cancer pain. Methods： Female Sprague-Dawley rats were divided randomly into the following groups： sham operation group, model group, Epimedium brevicornum group and Zoledronic acid group. Rats of sham operation group were Intra-tibial injected normal saline, while others were inoculated MRMT-1 cells to develop model of bone cancer pain. Pure water, Epimedium brevicornum and Zoledronic acid was given to different group respectively. On the nineteenth day after operation, ailodynia and hyperalgesia was tested. All rats were sacrificed on the twenty-first day, tibias were collected to measure the volume of tumors, quantitative measurement of bone mineral density （BMD） and bone mineral capacity （BMC）, then made into paraffin sections, through tartrate-resistant acid phosphatase （TRAP） stain to observe the bone pathological changes, count TRAP （＋） osteoclasts. Results： Compared to model group, allodynia and hyperalgesia was decreased （P 〈 0.01, P 〈 0.05）, BMD and BMC was enhanced （P 〈 0.01, P 〈 0.05）, osteoclast was decreased （P 〈 0.05） on Epimedium brevicornum group, and tumor volume was showed tendency to decrease, but no significance. Conclusion： Epimedium brevicornum showed effect toalleviate bone cancer pain induced by bone metastasis, which may be one of the mechanisms to block the excessive activation of osteoelasts.