摘要
目的:探讨痰瘀同治方对鸡胚绒毛尿囊膜血管新生的影响。方法:将8日龄鸡胚制备鸡胚绒毛尿囊膜(CAM)模型随机分为4组:空白血清组,大、中、小剂量含药血清组(加入各组相应的含药血清)。培养3d后观察各组对CAM模型标本形态及血管新生数量的影响。结果:大剂量含药血清组粗大血管及细小分支迅速增多,血管网络清晰,呈叶脉样,放射性生长,血管新生数量明显高于空白血清组(P<0.01),中剂量及小剂量含药血清组与空白血清组比较有一定优势,但无统计学意义。结论:痰瘀同治方可明显促进鸡胚绒毛尿囊膜血管新生。
Objective： To observe the effect of formula of Tan Yu Tong Zhi for promoting angiogenesis on chofio-auantoic membrance （CAM）. Methods： Prepare model of CAM, use medicated serum of the formula of Tan Yu Tong Zhi on CAM, The breeding eggs were divided into 4 groups： high-dose, medium-dose, low-dose and blank control serum. Growth shape of chicken embryo and vessels count of CAM model in each group were observed after 3 days. Results： The vasculature growth was rapidly in the medication group with high-dose. The primary and secondary vessels were promoted in the groups with high-dose, which was significantly different from normal serum group （P〈0.01）. There was no significant difference between the medium-dose and normal serum group. Conclusion： The fomula of Tan Yu Tong Zhi showed obvious promotion effects on CAM angiogenesis.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第5期1292-1294,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
重大新药创制(No.2009ZX09303-003
No.2009ZX09301-005-2-6)~~
