不同产地夏枯草药材HPLC特征图谱

HPLC fingerprint analysis of Prunella vulgaris from different habitat

目的:建立夏枯草药材高效液相色谱(HPLC)特征图谱分析方法,为科学评价夏枯草药材质量提供参考。方法:采用Diamonsil C18色谱柱(4.6mm×250mm,5μm),甲醇-0.1%的磷酸水溶液梯度洗脱,流速1.0mL/min,检测波长210nm,柱温:30℃。所得不同样品的特征图谱用相似度评价软件进行相似度分析,用SPSS进行聚类分析。结果:建立了夏枯草的特征图谱,分析了10个产地夏枯草药材的HPLC特征图谱,经相似度分析,确定了29个色谱峰为夏枯草药材特征峰,指认了其中的8个共有峰。结论:该方法简便、准确、重现性好,研究结果初步说明了不同产地夏枯草药材的差异与相似性,为夏枯草的质量评价和质量控制提供了参考。

Objective： To establish the method of fingerprint analysis on Prunella vulgaris by HPLC, which could be used for quality evaluation of herbs Prunella vulgaris. Methods： Column of Diamonsil C18 （4.6mm×250mm, 5μm） was adopted with a gradient elution composed of methanol- 0.1% phosphoric acid solution as a mobile phase at flow rate of 1.0mL/min and detection wavelength was 210nm, and column temperature was 30℃. Evaluated the similarity of the 10 samples by the software of ‘traditional medicine fingerprint similarity evaluation＇, and Cluster analysis has been carded out using SPSS. Results： Established the method of fingerprint analysis on Prunella vulgaris by HPLC, twenty-nine characteristic peaks were established in the fingerprint of 10 samples, among them 8 peaks were identified. Conclusion： The method is simple and accurate with good reproducibility. It can be used for the quality control of Prunella vulgaris.