细菌脂多糖对人外周血单核细胞来源的树突状细胞发育和功能的影响

EFFECTS OF LIPOPOLYSACCHARIDE ON THE MATURATION OF NOMRAL HUMAN DENDRITIC CELL

目的 :探讨细菌脂多糖 (LPS)对正常成人外周血单核细胞来源的树突状细胞 (Dendritic cell,DC)表面分子表达及免疫功能的影响。方法 :分离正常人 (n=5 )外周血单个核细胞 (PBMC)以 GM-CSF、IL-4联合诱生 DC,实验组在培养的第 2天加入LPS,正常对照组不加。于第 4、8天 FACS检测 DC表面分子 CD1a、CD14、CD83、CD80、HL A-DR的表达。同期检测 DC的增殖和刺激同种异体 T淋巴细胞的增殖情况。结果 :经 LPS刺激后 ,正常人 DC早期、晚期表面分子 CD1a、CD83、CD80表达增高 ,CD14表达降低 (P<0 .0 5 )。DC数量的增加及刺激同种异体 T细胞能力明显增强。结论 :GM-SF、IL-4和 L PS联合刺激能促进正常人

Objective: To determine whether bacterial Lipopolysaccharide(LPS) have effects on the phenotype and function of normal human dendritic cell (DC). Methods: Normal human DC were isolated from peripheral blood monounclear cells (PBMC) cultured in granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL) 4 . DC maturation was induced by LPS as experimental groups while the control groups underwent without LPS. DC phenotype was characterized by flow cytometric analysis and the immune function was detected through the stimulatory capacity of allogeneic lymphocytes on the 4th, 8th day of the culture. Result: Normal DC expressed CD1a , CD83 and costimulating molecules CD80 highly but CD14 lowly on cell surface by the stimulating of LPS(P<0.05). The quantity of DC and the ability of stimulating allogeneic T lymphocytes were also increased compared with the control groups. Conclusion: Normal human DC cultured in GM-CSF and IL-4 adding with LPS could increase the immune function and maturation of normal human DC. [