食用植物油中动物源性成分PCR检测方法的建立

Detection of Animal-derived Ingredients in Edible Vegetable Oils by PCR Method

本文建立了应用PCR技术快速鉴别食用植物油中动物源性成分的方法。以食用植物油中掺入的动物源性基因为靶标,自行设计16S rRNA基因通用引物,同时选用动物物种特异性引物进行PCR扩增,分别得到相应的目的片段。通过三对16S rRNA基因通用引物,建立了从食用植物油中快速检测是否含有动物源性成分的方法;利用所选的动物物种特异性引物,进一步建立了从食用植物油中鉴定猪、牛、羊、鸡、鱼5种动物源性成分的方法,此方法能检测出含有0.1%(m/m)动物源性成分的植物油。

In this article, a rapid and simple method of animal-derived ingredients in edible vegetable oils was established. Two pairs of universal primers to animal species and five pairs of specific primers to pig, bovine, sheep, chicken and fish wcrc designed for PCR amplification. The amplicons of universal primers and species-specific prirncrs wcrc used for animal-derived ingredients detection. Sensitivity and specificity of the newly developed method were confirmed with a detection level of 0.1% （m/m）.