颅内肿瘤148例免疫组化染色及随访研究

AN IMMUNOHISTOCHEMICAL AND FOLLOW-UP STUDY OF 148 INTRACRANIAL TUMORS

目的 :研究胶质纤维酸性蛋白 ( GFAP)和 S-10 0蛋白 ( S-10 0 )免疫组化染色在颅内肿瘤中的诊断价值。方法 :对 14 8例颅内肿瘤的手术切除标本进行常规外检取材 ,福尔马林固定 ,石蜡包埋 ,切片厚 5 μm,HE染色 ;对其连续切片用 GFAP和 S-10 0抗体进行免疫组化染色 ,跟踪随访 5 5例胶质瘤患者。结果 :GFAP存在于各级星形胶质细胞瘤、室管膜瘤、多形性胶质母细胞瘤、少枝胶质细胞瘤、混合性胶质瘤的星形细胞成份。S-10 0分布于颅内胶质来源的和非胶质来源的肿瘤。随着胶质瘤恶性程度的增加 ,GFAP免疫染色逐渐减弱 ( P<0 .0 0 1) ,2年生存率越低 ,预后越差 ( P<0 .0 0 1)。结论 :GFAP和 S-10 0有助于颅内肿瘤的诊断和鉴别诊断 。

Objective:To study the value of glial fibrillazy acidic protein(GFAP) and S-100 protein immunohistochemical staining to the diagnosis of the intracranial tumors.Methods:Resected tumor samples of 148 intracranial tumors were fixed in formalin and embedded in paraffin. The 5μm tumor sections were used for histology (hematoxylin and eosin stain),and consecutive sections were stained immunohistocchemically with GFAP and S-100 protein antibodies.55 gliomas were followed up.Result:GFAP was present in different varieties of astrocytomas,ependymoma,multiforme glioblastoma,eligodendroglioma,astrocytic elements of mixed gliomas.S-100 protein existed in both nonglial tumors and glial origin tumors.With the increase in malignancy of the glioma,the positive staining Yate of GFAP was decreased (P<0.001), the positive staining sate of GFAP was decreased (P<0.001),two-years survival rate was lower and the prognose was poorer (P<0.001).Conclusion:GFAP and S-100 immunohistochemical stainings are helpful to the diagnosis and differentiated diagnosis of incranial tumors.Detection of GFAP expression may have significant value in the accuracy of glial origin tumor and evaluation of the prognosis. [