HPLC法同时测定牙周康胶囊中甲硝唑和芬布芬的含量

Simultaneous determination of metronidazole and fenbufen in the Yazhoukang capsule by HPLC

目的 :建立测定牙周康胶囊中甲硝唑和芬布芬含量的方法。方法 :高效液相色谱法。固定相为十八烷基硅烷键合硅胶 ,流动相 :甲醇 0 .0 1mol/L十二烷基硫酸钠 ( 63∶37) (用磷酸调节pH值至 2 .5) ,检测波长为 2 95nm .结果 :同时测定甲硝唑和芬布芬的含量。甲硝唑平均回收率为 99.8% ,n =6,RSD为 0 .4 0 %。芬布芬平均回收率为1 0 0 .1 % ,n =6,RSD为 0 .36%。结论 :本方法简便、快速、可靠 。

OBJECTIVE:To establish a method for determination of metronidazole and fenbufen in the Yazhoukang capsule.METHODS:The HPLC method empolyed a C 18 column with the mobile phase methanol 0.01mol/L sodium dodecyl sulphate(63∶37)(adjusted the solution with phosphoric acid to pH 2.5).The detection wavelength was set a 295nm.RESULTS:The content of metronidazole and that of fenbufen was assaged respectively.The average recovery for metronidazole was 99.8%(n=6),RSD=0.40%,and the average recovery for fenbufen was 100.1%(n=6),RSD=0.36%.CONCLUSION:The method is simple,fast and reliable.It was a good method for the determination of metronidazole and fenbufen in the Yazhoukang capsule.