早春短命植物绵果荠愈伤组织诱导相关因素研究

The relevant factors of callus induction of early spring ephemeral Lachnoloma lehmannii(Brassicaceae)

绵果荠是分布在新疆北部准噶尔荒漠中的十字花科一年生早春短命植物。本研究为探讨绵果荠愈伤组织诱导条件,以无菌苗子叶为外植体,研究了不同苗龄、培养基种类、蔗糖浓度和培养条件对愈伤组织的诱导及其生长的影响。结果表明,1)愈伤组织诱导的最佳条件为:苗龄14d、培养基为MS+1.0mg/L IBA+0.1mg/L BAP、含糖量3%、温度25℃和光照为黑暗。2)愈伤组织生长的适宜条件是光照16h/d和温度25℃。

Lachnoloma lehmannii is an annual early spring ephemeral Brassicaceae species in the Junggar Desert of northern Xinjiang. The primary aims of this study were to research the conditions for callus induction in L. lehmannii. Using the aspect seedling cotyledon as explants, the effect of seedling age, media, sucrose concen- tration and the culture condition on callus induction and their growth was investigated. The most suitable con- dition for callus induction was seedlings of optimal age （14 days） on an MS culture medium containing 1.0 mg/L IBA＋0.1 mg/L BAP, with a suitable concentration of sucrose （3%）, at a temperature of 25℃ in the dark. The best condition for callus growth was a temperature of 25℃ in the light with a photoperiod of 16 h/day.