转红树总DNA紫花苜蓿T_1代耐盐株系的生理生化特性分析

Analysis of physiological and biochemical characteristics of T1 salt-tolerant transgenic alfalfa transformed with the Rhizophora apiculatatotal total DNA via the pollen-tube pathway

以花粉管通道法转红树总DNA紫花苜蓿阿尔冈金T1代耐盐植株幼苗为材料,经300mmol/L NaCl处理后,测定叶绿素、脯氨酸、丙二醛含量和抗氧化酶活性等耐盐相关生理生化指标。结果显示,转基因株系叶绿素含量下降0.09～0.29倍,而对照植株下降0.32倍;转基因株系脯氨酸含量升高1.2～4.2倍,对照植株的脯氨酸升高0.9倍;转基因株系丙二醛含量升高0.15～0.29倍,对照植株的丙二醛含量升高0.36倍;转基因株系超氧化物歧化酶活性升高0.20～0.25倍,对照植株的超氧化物歧化酶活性升高0.19倍;转基因株系过氧化物酶和过氧化氢酶活性分别是对照的1.6～5.6倍和1.2～1.3倍。转基因株系表现出耐盐植物的生理生化特性。

With 300 mmol/L NaC1 stress, the physiological and biochemical indexes, including chlorophyll, proline and MDA content, and antioxidase activity, of T1 transgenic alfalfa transformed with Rhizophora apic- ulata total DNA by the pollen-tube pathway, were investigated. The transgenic plantlets had a reduced chloro- phyll content of 0.09-0.29 times, but the non-transgenic plant had 0.32 times. The proline content of trans- genic plantlets was enhanced by 1.2 to 4.2 times, but for non-transgenic plant it was 0.9 times. The increased proportion of MDA content was 0. 15--0.29 times in transgenic plantlets compared with 0.36 times for non- transgenic plant. However, the transgenic plantlets had increased SOD activity （0.20-0.25 times） compared with the non-transgenic plant （0.19 times）. The POD and CAT activity of the transgenic plantlets were 1.6-5.6 times and 1.2-1.3 times respectively compared with non- transgenic plants. This suggested that the trans- genic plantlets had the physiological and biochemical characteristics of salt tolerant plants.