盐胁迫对碱地风毛菊苗期PM-ATPase及5′-AMPase活性的影响

Effects of salt stress on the activity of PM-ATPase and 5′-AMPase of Saussurea runcinata at seedling stage

盐胁迫是影响植物生长、发育以至产量的重要因素,试验通过对碱地风毛菊苗期分别以NaCl和Na2CO3进行胁迫,Na+浓度为0(CK),60,120,180,240,300,360mmol/L,测定其丙二醛、PM-ATPase活性及5′-AMPase活性,以探讨盐胁迫对其膜系统的影响及其苗期耐盐性。结果表明,盐胁迫下,碱地风毛菊丙二醛含量增大,Na2CO3胁迫下,碱地风毛菊根系和叶片中MDA含量在Na+浓度为360mmol/L时达到最大值,分别为对照的2.15和1.94倍;在NaCl胁迫下,碱地风毛菊叶片中MDA含量最大为对照的1.22倍。同一盐胁迫下,碱地风毛菊根系中PM-ATPase活性和5′-AMPase活性显著高于叶片(P<0.05),碱地风毛菊根系中PM-ATPase活性最大为56.29μg磷酸/(mg蛋白.h),而5′-AMPase活性最大为53.91μg磷酸/(mg蛋白.h)。2种盐胁迫下PM-ATPase和5′-AMPase活性差异显著。总之,盐胁迫下,碱地风毛菊根系比叶片受到的损害小,碱地风毛菊更耐NaCl胁迫。

Salt stress affects plant growth and development and reduces productivity of crops. This experiment was carried out to determine the MDA, plasma membrane ATP enzyme （PM-ATPase） and 5＇-neucleotidase （5＇-AMPase） of Saussurea runcinata seedling by NaC1 and Na2COa stress （The concentration of Na＋ is 0, 60, 120, 180, 240, 300 and 360 retool/L） in order to assess the effects of its membrane systemthe under salinity and salt tolereanee about it. It has been shown that the MDA increased by salinity stress. The content of MDA in roots and leaves of S. runcinata reached the maximum under NazCOa stress in the Na＋ concentration of 360 mmol/L, respectively, 2.15 times and 1.94 times than the control. The maximum content of MDA in leaves of S. runcinata 1.22 times than the control. The activity of PM-ATPase and 5＇-AMPase in the roots is higher than those in the leaves under the same concentration of Na＋. The activity of PM-ATPase and 5＇-AMPase were significantly difference by two different salinity stress. The maximum activity of PM-ATPase in the roots of S. runcinata up to 56.29μg pi/（mg protein·h） ,but the activity of 5＇-AMPase up to 53.91 /,g pi/（mg protein·h）.