RP-HPLC法测定金嗓散结丸中丹参酮ⅡA的含量

Determination of Tanshinone ⅡA in Jinsang Sanjie pills by RP-HPLC

目的建立一种可测定金嗓散结丸中丹参酮ⅡA的含量的RP-HPLC方法。方法采用岛津VP-ODS(150mm×4.6mm,5μm)为色谱柱;流动相为乙腈-水(75∶25);检测波长为270nm;流速为1.0mL·min-1;峰面积外标法定量;结果本法丹参酮ⅡA在0.37μg·mL-1～36.74μg·mL-1范围内呈良好的线性关系,(r=0.99994)平均回收率分别为99.9%;RSD1.7%;结论方法简便,快速,准确可靠,可作为该制剂的质控方法。

OBJECTIVE To establish an HPLC method for determining the content of Tanshinone ⅡA in Jinsang Sanjie pills.METHODS Shimadzu VP-ODS（150mm×4.6mm,5μm） was used,Acetonitrile-water（75∶25）was used as mobile phase.The detection wavelength was 270nm and the flow rate was 1.0mL·min-1.External standard method was used.RESULTS TanshinoneⅡA concentration presented a good linear range of 0.37μg·mL-1～36.74μg·mL-1（r=0.9999）,the average recovery was 99.9%,RSD=1.7%.CONCLUSION The method is simple,rapid and accurate for quality control of the preparation.