摘要
建立了黄芪颗粒中总黄酮的含量测定方法:以芦丁为参照,利用紫外分光光度法在371 nm波长测定黄芪颗粒中总黄酮的含量。芦丁在5.90～59.0μg/mL线性关系良好,线性方程为A=0.0103C+0.2707,γ=0.9963,平均回收率为98.65%,RSD%=0.85%。结果表明:紫外分光光度法测定黄芪颗粒中总黄酮的含量方法简便,快捷,准确,可用来做黄芪颗粒的质量评估的方法。
A new method for determination the total flavonoids in Huangqi Granule was established. The total fla- vonoids in Huangqi Granule were determined by UV spectrophotometry using rutin as the reference at the wavelength of 371 nm. The linear equation was A = 0.0103C ＋ 0.2707, γ/= 0.9963, and linear range of calibration curve was 5.9 59 μg/mL. The average recovery rate was 98.65% with the relative standard deviation 0.86%. The method of UV spec- trophotometry determination the total flavonoids in Huangqi Granule was simple, fast and accurate, which can be used to evaluate the quality of Huangqi Granule.
出处
《广州化工》
CAS
2012年第8期133-134,142,共3页
GuangZhou Chemical Industry
基金
贵州省教育厅自然科学研究项目(黔教科(2011)026号)
贵阳市科学技术计划项目(筑科合同[2011204]24号)
贵阳市科学技术计划项目[2010]筑科计合同字第3-重-1号
贵州省技术创新项目(2011040)
贵州省科技创新人才团队建设项目黔科合人才团队(2011)4008)
关键词
黄芪颗粒
总黄酮
紫外分光光度法
Huangqi Granule
total flavone
UV spectrophotometry
