清醒小鼠反复脑缺血再灌注法致学习记忆障碍模型的建立

Model of dysmnesia by repeated cerebral ischemia-reperfusion in conscious mouse

目的 建立一种清醒小鼠反复脑缺血再灌注致学习记忆障碍模型的方法。方法 采用清醒小鼠反复脑缺血再灌注及单次脑缺血再灌注手术方法 ,应用跳台法 ,避暗法 ,脑海马CA1区神经细胞计数等方法研究脑缺血再灌注对小鼠行为学 ,脑病理形态学的影响 ,并预防应用尼莫地平等药物研究了反复脑缺血再灌注造模方法的稳定性及可靠性。结果 与假手术组比较 ,反复脑缺血再灌注使小鼠出现明显的学习记忆功能障碍且比相同缺血时间的单次脑缺血再灌注造成的损伤更为明显 ,并使脑海马CA1区神经细胞数明显减少 ;尼莫地平等药物可不同程度地改善反复脑缺血再灌注致学习记忆障碍小鼠的学习记忆功能。结论 清醒小鼠反复脑缺血再灌注法是一种稳定可靠 ,简便易行的致学习记忆障碍的造模方法。

AIM To develop a model of dysmnesia by repeated cerebeal ischemia reperfusion in conscious mouse METHODS Repeated and single cerebral ischemia reperfusion in conscious mouse were established On these models in mouse, influence of cerebral ischemia reperfusion on behavior and pathomorphology were studied by step down test、step through test and neurons determination of hippocampas CA 1 Nimodipine and extracts of gingkgobiloba leaves were also used to identified the stability and reliability of this model RESULTS Repeated cerebral ischemia reperfusion showed more severe impairment of learning and memory than single ischemia reperfusion compared with Sham In the mean time ,neurons of hippocampus CA 1 were damaged,nimodipine and extracts of ginkgobiloba leaves could improve the impairment of learning and memory in mouse in different levels CONCLUSIONS The method of repeated cerebral ischemia reperfusion has been shown to be well suitable for dysmnesia model in conscious mouse because of its easy、reliable and simple precedure of application