HT1080R细胞的辐射抗性与细胞周期关系初步研究

Radioresistance and cell cycle distribution of HT1080R cells after γ-ray irradiation

目的 研究HT10 80R的辐射抗性及其照射前后细胞周期分布 ,探讨其辐射抗性的分子机理。方法 克隆形成比较HT10 80与HT10 80R照射后细胞存活率 ,流式细胞术研究细胞周期分布 ,PCR SSCP和Western杂交测定 p5 3外显子的基因状态及其表达状况。 结果 HT10 80R的辐射敏感性降低 ,具有明显的辐射抗性。照射后具有G2 期阻滞现象 ,而其p5 3基因 5个外显子正常。结论 经γ射线照射HT10 80R细胞周期改变与其亲本细胞明显不同 ,出现明显的G2 期阻滞 ,提示HT10 80R辐射抗性的获得可能与其细胞周期G2 期阻滞有关 ,PCR SSCP和Western杂交研究表明HT10 80R细胞辐射抗性不是由于 p5 3基因功能异常所致。

Objective\ To investigate radioresistance and cell cycle distribution of HT1080R cells after γ ray irradiation. Methods By comparison of the survival fraction between HT1080R and HT1080 using cloning assay,cell cycle distribution and gene status were investigated by FCM and PCR\|SSCP,respectively. Results HT1080R was more radioresistant than its parental cell line HT1080,and after exposure to 4 Gy and 6 Gy γ \|rays showed G 2 phase arrest.PCR\|SSCP showed that the expression of p53 gene was normal. Conclusion \ G 2 arrest can be found after 4 Gy and 8 Gy γ ray irradiation in HT1080R cell line,which is more radioresistant than its parental cell line HT1080,suggesting that the acquirement of radioresistance may be related to its G 2 arrest.Because of its normal gene status and expression,radioresistance of HT1080R cannot be explained by abnormal function of p53 gene.