NK及B细胞水平对肝癌家族聚集性影响的研究

The Study of the Impact of Natural Killer Cells and B Cells on the Familial Aggregation of Hepatocellular Carcinoma

目的探讨免疫细胞NK细胞和B细胞水平对肝癌家族聚集性的影响。方法以广西肝癌高发区肝癌高发家族成员94人作为观察组,选取年龄±5岁,性别、民族相同,生活环境、生活水平及生活习惯相似,居住距离1 km以内的无癌原籍居民94人作为对照组。应用流式细胞仪检测两组外周血中NK细胞和B细胞。结果观察组NK细胞为(15.03±7.05)%,对照组为(16.33±7.42)%,而B细胞为(6.27±2.72)%,对照组为(5.90±2.52)%,两组NK细胞、B细胞比率差异均无统计学意义(P>0.05);观察组NK细胞/B细胞比值为2.76±1.69,显著低于对照组的3.46±2.84,差异有统计学意义(P<0.05)。结论 NK细胞/B细胞比值显著下降导致机体免疫功能失调,可能促进肝癌的发生,并导致肝癌的家族聚集性。

Objective To investigate the effect of natural killer cells（NK cells） and B cells on the familial aggregation of hepatocellular carcinoma.Methods Ninety four familial members（observation group） with hepatocellular carcinoma in regions of high hepatocellular carcinoma incidence rate in Guangxi and 94 healthy controls（control group） matched with the same nationality,gender,about ±5 years of age,and residential area were selected.The levels of NK cells and B cells in the peripheral blood from the subjects were tested by flow cytometry.Results The level of NK cells in observation group was（15.03±7.05）%,while in control group was（15.03±7.05）%;the level of B cells in observation group was（6.27±2.72）%,while in control group was（5.90±2.52）%,there was no significant difference between the two groups in the level of NK and B cells（P0.05）.The ratio of NK/B cells in observation group was 2.76±1.69,while in control group was 3.46±2.84,there was significant difference between the two groups（P0.05）.Conclusion The significant reduction of the ratio between NK cells and B cells in the body might cause dysfunction of immune system,promote tumor development and induce familial aggregation of hepatocellular carcinoma.