肺泡巨噬细胞CD11b/CD18及其配体CD54的表达与肺泡炎症的相关性

Correlation of the Expression of CD11/CD18 Intercellular Adhesion Molecule and its Ligand CD54 with Alveolar Inflammation

目的 :通过博莱霉素致大鼠肺纤维化过程中肺泡巨噬细胞表面白细胞粘附分子 CD11b,CD18及相应配体 CD5 4表达的动态观察 ,研究白细胞粘附分子对肺泡炎的触发作用。方法 :采用流式细胞仪和免疫组化方法。结果 :肺泡巨噬细胞 CD11b,CD18的表达在博莱霉素灌注后 1d即开始增高 ,7～ 14d天达高峰 ,与对照组差异显著(P <0 .0 0 1) ,随后略下降 ,但仍处于高水平表达。而 CD5 4在 3d后表达明显升高 ,以后呈持续性高水平表达。且7d时 CD11b,CD5 4的表达与 BAL F中的肺泡巨噬细胞 (Alveolar macrophage,AM)的数量呈正相关 ,γ值分别为0 .995 ,0 .885。两周后 BAL F中的肺泡巨噬细胞数量恢复正常 ,而其表面 CD11b,CD18,CD5 4的表达仍然增强。结论 :博莱霉素致大鼠肺纤维化发生机制中 CD11b,CD18,CD5 4表达增强 ,其在触发肺泡炎症及介导肺纤维化过程中起一定的作用。

Objective:To investigate the tiggering role of CD11/CD18 leukocyte surface adhesion molecule and its ligand CD54 to alveolar inflammation during the course of pulmonary fibrosis induced by Bleomycin on alveolar macrophages (AM) and evaluating the relationship between the expression of CD11b / CD18 and recruitment of alveolar macrophage(AM) in the BALF.Methods:We use immunofluorescence flow cytometry and immunohistochemistry method to determine the expression of CD11b,CD18,CD54 on AM Results:(1)The expression of CD11/CD18 increased on the first day,peaked on 7 14 th days(P<0.001) and subsequently decreased lightly, but remained at high level compared with those of the control.(2)The imcreased expression of CD54 began on the 3rd day(P<0.01) and maintained at high level.(3)The expression of the CD11b,CD54 was greatly correlated with the number of the AM on 7th days(r=0.995,r=0.885).(4)The expression of CD11b, CD18, CD54 was still very high after 2 weeks,but the number of the AM in BALF had declined to normal level.Conclusion:The increased expression of CD11b,CD18,CD54 on AM in Bleomycin induced pulmonary fibrosis may play an important role in tiggering alveolar inflammation and in inducing lung interstitial fibrosis.