自体移植静脉平滑肌细胞凋亡与增殖关系的实验研究

The Relationship Between Apoptosis and Proliferation of Smooth Muscle Cell in Autogenous Vein Grafts

目的 :探讨移植静脉狭窄的机制。方法 :建立 10 0只 Wistar大鼠自体颈静脉移植于肾下腹主动脉模型。应用 DNA原位缺口末端标记及免疫组织化学 SP法 ,检测术后 1～ 8周移植静脉平滑肌细胞的凋亡和增殖细胞核抗原的表达。结果 :术后 1～ 8周移植静脉平滑肌细胞凋亡及增殖均高于对照静脉 (P <0 .0 1) ;术后 1～ 2周 ,凋亡低于增殖 ,术后 4～ 8周凋亡高于增殖 ,凋亡与增殖具有相关性 (r=0 .813,P <0 .0 5 )。结论 :移植静脉平滑肌细胞凋亡和增殖失衡与移植静脉的狭窄有关 ,防治移植静脉狭窄应调节增殖与凋亡的平衡。

Objective:To study the relationship between apoptosis and proliferation hence to explore the mechanism of vein stenosis after autogenous vein grafts. Methods:A rat experimental model of autogenous vein graft was established by transplanting the right external jugular vein into infrarenal abdominal aorta in 100 Wister rats. Immunohistochemical S P technique and terminal transferase dUTP biotin nick end labelling(TUNEL) were used to detect the expression and distribution of proliferation cell nuclear antigen(PCNA) and apoptosis of smooth muscle cells(SMCs) in vein graft at different time from 1 to 8 weeks. Results:From 1 to 8 weeks after replacement, the expression of apoptosis and PCNA of SMCs in experimental group was continuously higher than those of the control groip(P<0,01). In 1 to 2 weeks, the positive rate of TUNEL was lower than that of PCNA. But from 4 to 8 weeks, the positive rate of TUNEL was higher than that of PCNA. The expression on apoptosis correlated with PCNA(r=0.813,P<0.05). Conclusion:The imbalance of proliferation and apoptosis may relate to the vein graft stenosis, and regulating the balance between proliferation and apoptosis may be useful to prevent vein graft stenosis.