重型病毒性肝炎经输血传播病毒感染状况调查及其临床意义

Detection of Transfusion Transmitted Virus Infection and Clinical Investigation Among Patients with Fulminant Hepatitis

目的 :调查重型病毒性肝炎经输血传播病毒 (transfusion transmitted virus,TTV)的感染状况。方法 :设计 TTV基因的特异性引物 ,采用巢式 PCR法对 37例重型肝炎进行血清中 TTV DNA的检测 ,并对 TTV DNA阳性病例进行临床资料分析。结果 :37例重型肝炎中 9例血清 TTV DNA阳性 (2 4.3% ) ,其中 7例重叠感染 HBV,1例重叠感染 HCV,1例为单纯 TTV感染 ;9例中急性重型肝炎 1例 ,亚急性重型肝炎 6例 ,慢性重型肝炎 2例 ;9例中 5例死亡 (5 5 .6 % ) ,1例有明确输血史。结论 :TTV可在各型重型肝炎外周血中检出 ,TTV可能是导致重型肝炎和促进肝功能衰竭的重要因素之一。

Objective: To investigate the presence of transfusion transmitted virus (TTV) infection among patients with fulminant hepatitis. Methods: Total of 37 serum samples from patients with fulminant hepatitis were detected for TTV DNA by nested PCR methods. Results: The positive rate of TTV infection among patients with fulminant hepatitis was 24.3% (9/37). Among nine TTV DNA positive patients, seven were coinfected with HBV, one was coinfected with HCV, one was only TTV infection. Conclussion: TTV infection is associated with fulminant hepatitis, only TTV infection and coinfection with other hepatitis virus would be the important cause of fulminant hepatitis and hepatic failure.