半夏总生物碱对帕金森病大鼠的学习记忆及氧化应激反应的影响

Eeffects of total alkaloids from Pinellia ternate on learning,memory and oxidative stress in Parkinson's disease rat models

目的探讨半夏总生物碱(total alkaloids from Pinellia Ternate,TAPT)对帕金森病模型大鼠的防治作用及其抗氧化机制。方法采用脑内定位注射6-羟基多巴胺(6-OHDA)建立帕金森病大鼠模型,在模型建立成功的同时给予半夏总生物碱预防性治疗。采用Morris水迷宫进行帕金森病大鼠的行为学检测,用化学比色法检测大脑皮质及血清超氧化物歧化酶(SOD)活力、丙二醛(MDA)、过氧化氢(H2O2)和还原型谷胱甘肽(GSH)的含量。结果与正常组比较,帕金森病模型组大鼠的逃避潜伏期明显延长(P<0.01),跨越平台次数明显减少(P<0.01);经半夏总生物碱治疗组,大鼠逃避潜伏期明显缩短(P<0.05,P<0.01),跨越平台次数明显增加(P<0.01)。帕金森病模型组大鼠脑皮质及血清中MDA、H2O2的含量增加,SOD活性及GSH的含量降低(P<0.01);经半夏总生物碱治疗组,脑皮质MDA、H2O2的含量显著减少(P<0.01),皮质GSH、SOD含量显著增加(P<0.01);半夏总生物碱给药组中低浓度组、中浓度组血清MDA的含量无统计学意义(P>0.05),高浓度组血清MDA含量下降(P<0.01),各治疗组中血清H2O2含量明显下降(P<0.01),血清GSH含量显著增加(P<0.01)。结论半夏总生物碱具有改善学习记忆能力,对抗大鼠神经系统的退行性变有一定的作用,可能通过改变帕金森病模型大鼠皮质部分及血清SOD、GSH的含量,而抑制了MDA和H2O2的产生。

Objective To explore the treatment and anti-oxidation mechanism of total alkaloids from Pinellia ternate（TAPT） in Parkinson＇s disease（PD） rat models.Methods The rat model of Parkinson＇s disease was established by injection of 6-hydroxydopamine（6-OHDA） into the rat brain.TAPT was given to the model rats to observe its preventive effect.The behavioral changes of the PD rats were tested with Morris water maze.Chemical assay was used to measure the superoxide dismutase（SOD） activity,malondialdehyde（MDA）,hydrogen peroxide（H2O2） and glutathione（GSH） contents in the cerebral cortex and serum.Results Compared with the normal group,the escape latency of the PD rats was significantly longer（P0.01）,the number of platform crossing significantly reduced（P0.01）.After TAPT treatment,the escape latency of PD rats was significantly shorter（P0.05,P0.01）,and the number of platform crossing was significantly increased（P0.01）.In the Parkinson model group,the MDA and H2O2 contents were increased,the SOD and GSH levels in serum and cerebral cortex were significantly decreased（both P0.01）,the MDA and H2O2 contents were significantly reduced（P0.01）,and the GSH and SOD levels in the cerebral cortex were significantly increased（P0.01）.The serum MDA levels in the low concentration and middle concentration TAPT treatment groups were not significantly changed（P 0.05）,but significantly decreased in the high concentration TAPT treatment group（P0.01）.The serum H2O2 in the treatment groups was significantly decreased（P0.01）,and serum GSH contents in the treatment groups significantly increased（P0.01）.Conclusions TAPT treatment show improving effect on learning and memory in PD rats,and may play a role against the degeneration in the rat nervous system,possibly through changes in the cortical and serum SOD,GSH levels,while inhibit of MDA and H2O2 generated in the rats with Parkinson＇s disease.