唾液酸受体并非流感病毒各亚型在雪貂组织中播散分布的决定因子

Sialic Acid Receptors Are not Determinant Factors for the Organ Distribution of Different Subtype Influenza Viruses in Ferret

目的探讨流感病毒在雪貂组织中的分布与唾液酸受体的关系。方法用病毒分离的方法分析流感病毒H5N1(SZ406H,A/VN/1203/04),SH1N1,H3N2(Brisbane/09,HK/09)在雪貂各组织中分布,用直接免疫荧光法分析雪貂各组织的唾液酸受体的分布,并通过体外实验证实活病毒与组织上受体的结合。结果 H5N1(SZ)和H5N1(A/VN/1203/04)在雪貂的肝、脾、肺、肠中有分布,H5N1(A/VN/1203/04)在脑组织中也有分布,而SH1N1、H3N2(Brisbane/09,HK/09)只分布于肠组织。而唾液酸受体SAα2,6Gal和SAα2,3Gal的I型受体分布于脾、心、肺、肠、脑组织中,和SAα2,3Gal II型受体分布于肝、脾、心、肺、肠、脑组织。SH1N1病毒与SAα2,6Gal能结合,而H5N1与SAα2,3Gal结合。结论 H5N1能在雪貂的多器官组织组织中分布和繁殖,而H3N2和SH1N1仅能在肠组织中分布繁殖。SAα2,6Gal和SAα2,3Gal受体在雪貂多器官组织中均有表达,说明唾液酸受体是病毒进入的门户,但不是病毒分布的决定因子。

Objective To investigate the relationship between tissue tropism of influenza viruses and sialic acid receptors in ferret.Methods The distribution of influenza viruses H5N1（SZ406H,A/VN/1203/04）,SH1N1,H3N2（Brisbane/09,HK/09） in infected ferrets was analyzed by virus isolation,and the distribution of sialic acid receptors in tissues was determined by direct immunofluorescence assay.The binding of influenza virus to sialic acid receptor was detected by indirect immunofluorescence assay.Results H5N1（SZ） and H5N1（A/VN/1203/04） viruses were detected in the liver,spleen,lung and intestine.In addition,H5N1（A/VN/1203/04） virus was also detected in the brain.SH1N1 and H3N2（Brisbane/09,HK/09） viruses were detected in the intestine.Sialic acid receptors type I SAα2,6Gal and SAα2,3Gal were distributed in the spleen,heart,lung,intestine,and brain,type II SAα2,3Gal receptors were distributed in the spleen,heart,lung,intestine,brain,and liver.SH1N1 virus was bound to SAα2,6Gal in the tissues,while H5N1 was bound to SAα2,3Gal.Conclusions Our results indicate that H5N1（SZ,VN） virus can be distributed in many organs in ferret.SH1N1 and H3N2（HK,BR） viruses are only distributed in the intestine,while both SAα2,3Gal and SAα2,6 Gal receptors are distributed in many organs.So that we would conclude that SA receptors are not determinant factors for organ distribution of different subtype influenza viruses in ferrets.