云南地区恒河猴线粒体DNA控制区遗传多态性研究

Genetic Polymorphism of Mitochondrial DNA Control Region in Macaca mulatta from Yunnan Province

目的从分子水平探讨云南地区恒河猴遗传多样性,为今后开展恒河猴遗传资源的保护及合理利用提供借鉴和背景资料。方法采用PCR直接测序法测定云南地区恒河猴96份样品的线粒体DNA控制区全序列,用Mege 4.0和DNA SP软件对变异位点数、简约信息位点数、单倍型、单倍型多样度、核苷酸多样度等遗传信息进行分析,基于邻接法(neighbor-joining,NJ)和最小进化法(minimum-evolution,ME)构建系统发生树。结果在96份样品中,共检测出了149个多态性位点,定义了46种单倍型,单倍型多样度(Hd)为0.968±0.007,核苷酸多样度(Pi)为0.020。结论云南地区恒河猴存在着较丰富的遗传多态性。

Objective To analyze the genetic diversity and phylogenetic of Macaca mulatta in Yunnan Province,which provide important reference and background data for researches of macaca mulatta protection and reasonable application.Methods Ninety six blood samples of Macaca mulatta were collected for amplifying the complete sequences of mitochondrial DNA control region by polymerase chain reaction（PCR）.The variation sites,parsimony informative sites,haplotypes,haplotype diversity,and nucleotide diversity were analyzed using Mega 4.0 and DNA SP software.The phylogenetic tree was constructed by neighbor-joining（NJ） and minimum-evolution（ME） methods.Results One hundred and forty-nine variation sites and 46 haplotypes were found in the ninety-sixth Macaca mulatta,the haplotype diversity（Hd） was 0.968±0.007 and nucleotide diversity（Pi） was 0.020.The phylogenetic analysis based on mitochondrial control region showed that these Macaca mulatta lived in Yunnan was divided into two sister groups geographically.Conclusions The results of this study indicate a high variation and phylogenetic evolution relationship of Macaca mulatta in Yunnan province.