人脑胶质瘤干细胞的分离和脑瘤浸润动物模型的建立

Isolation of human glioma-derived stem cells and establishment of an invasive glioma model

目的探讨利用脑胶质瘤分离培养胶质瘤干细胞的方法,并建立脑胶质瘤浸润动物模型。方法采用神经干细胞培养方法对12例手术切除的脑胶质瘤新鲜肿瘤组织进行培养,对形成细胞球的干细胞进行增殖、自我更新及分化试验,用免疫细胞化学法检测干细胞标志物,并用转染增强型绿色荧光蛋白的干细胞进行体内成瘤试验。结果 12例胶质瘤标本中共有9例成功培养出肿瘤神经细胞球,这些神经细胞球具有增殖、自我更新及分化能力,能表达特异性的分子标记物,并且可在免疫缺陷小鼠脑内形成与人类胶质瘤相似的、具有浸润性特征的肿瘤。结论采用神经干细胞无血清培养方法可以成功培养出胶质瘤干细胞,并且在体内建立了一种新的胶质瘤浸润模型。

Objective To isolate brain tumor stem cells（BTSCs）from glioblastoma（GBM）specimens,and to develop an infiltrative GBM model.Methods Glioma neural spheres were isolated from 12 glioma tissues.The cells were observed for proliferation,self-renewal and differentiation.Confirmation was done by testing for expression of specific molecular markers.These cells＇ functionality in initiating tumors was examined in immune-deficit mice.Results Glioma neural spheres were isolated from 9 out of the 12 samples and were able to express specific molecular markers.These cells had the capacity of proliferation,self-renewal and differentiation.Further,these cells were found to be able to initiate GBM-like tumors in mice brains.Conclusion We successfully isolated BTSCs from human glioblastoma（GBM） samples.An invasive glioma model was established.