抑瘤素M在膝关节骨关节炎中的表达

Expression of oncostatin M in knee osteoarthritis

目的检测抑瘤素M(OSM)在骨关节炎(OA)和正常滑膜组织中的表达差异,探讨OSM在骨关节炎发病中的作用。方法术中留取33例OA严重病变组、10例OA轻微病变组、8例正常对照组的滑膜组织。采用实时定量PCR技术检测各组膝关节滑膜组织中OSM的表达,免疫组织化学法检测各组滑膜组织中OSM的分布与表达。结果在膝关节滑膜组织中,OA严重病变组、OA轻微病变组和正常对照组均能检测出OSM阳性表达且与病情严重程度呈正相关,差异有统计学意义(P<0.05)。OA轻微病变组与对照组比较差异无统计学意义(P>0.05)。结论 OA患者滑膜细胞高表达OSM与病情相关。OSM在骨关节炎的发病过程中起到重要作用。

Objective To investigate the difference of OSM expression between OA and the normal synovial membrane,and to discuss its function in the pathogenesis of knee osteoarthritis.Method OSM expression was concurrently detected in synovial fluid samples from 33 patients with severe OA,10 patients with slight OA and 8 patients with normal disciform meniscus.We performed quantitative real-time PCR to determine all the genual synovium OSM gene expression levels.The distribution of OSM in all genual synovium was detected by immunohistochemical technology.Results OSM was found in all patients′ synovial fluid.The expression level of OSM had a positive correlation with the severity of OA（P〈0.05）.There was no significant difference in expression of OSM between patients with slight OA and patients with normal disciform meniscus（P〉0.05）.Conclusion OA synovial cells may have a higher level of OSM,which is related to the course of disease.OSM plays an important role in the pathogenesis process of osteoarthritis.