响应面法优化里氏木霉Rut C-30产纤维素酶液体培养基

Optimization of liquid-state fermentation medium for cellulase production by Trichoderma reesei Rut C-30 with response surface methodology

该试验在单因素对里氏木霉(Trichoderma reesei)Rut C-30产纤维素酶的液体培养基优化的基础上,以滤纸酶活为响应值,采用响应面法确定其最佳培养基。首先通过Plackett-Burman(PB)设计筛选出影响滤纸酶活的显著因素,结晶纤维素和麸皮;通过最陡爬坡试验逼近最大酶活力区域;最后通过Central Composite Design(CCD)设计及响应面分析确定产酶最佳培养基,其中影响酶活的显著性因素结晶纤维素41.8g,麸皮19.1g。经过优化,滤纸酶活力最高为8.21U/mL,比单因素优化结果7.03U/mL提高了16.78%,同时测得CMC酶活为63.64IU/mL,木聚糖酶活为27.4IU/mL,葡萄糖苷酶酶活0.96IU/mL。

Using filter paper activity（FPA） as response values,response surface methodology was used to optimize liquid-state fermentation medium for cellulase production by Trichoderma reesei Rut C-30.Plackett-Burman design was further adopted to screen the important factors affecting FPA,which were Avicel and wheat bran.The steepest ascent experiment was used to approach the optimal region of the highest FPA.The optimal fermentation medium obtained by Central Composite Design and response surface analysis was as follows： Avicel 41.8g/L,wheat bran 19.1g/L.Based on the optimized medium,FPA reached 8.21U/mL,which was increased by 16.78% when compared with that（7.03IU/ml） before optimization.