呼吸道合胞病毒感染大鼠肺实验模型的建立

Establishment of bronchiolitis model by respiratory syncytial viral infection in rats

目的建立动物毛细支气管炎模型。方法14只SD大鼠被分成对照组、模型组两组，大鼠在戊巴比妥钠腹腔注射麻醉下用呼吸道合胞病毒滴鼻吸入感染的方法建造病毒感染模型。采用荧光免疫试剂检测法检测大鼠血清白三烯C4（LTC4）、肿瘤坏死因子α[（TNF-α）、嗜酸细胞阳离子蛋白（ECP）、免疫球蛋白E（lgE）、白细胞介素-4（IL-4）、干扰素-γ（INF-γ）含量。采用多道生理信号采集处理系统测大鼠气道阻力及肺顺应性。结果与对照组比较，模型组大鼠血清LTC4、TNF-α、ECP、lgE、IL-4均升高（P〈005或0．01）：与对照组比较，模型组气道阻力明显升高（P〈0．01），肺顺应性明显下降（P〈001）。结论滴鼻吸入呼吸道合胞病毒悬液法能成功建立动物毛细支气管炎模型。

Objective To establish an animal model of bronchiolitis. Methods Fourteen SD rats were randomly divided into two groups： the normal control group and bronchiolitis model group. Rats were anesthetized by intraperitoneal injection of pentobarbite; then the respiratory syncytial virus （RSV） was dropped in nasal cavity. The serum levels of LTC4, TNF ~, ECP, IgE, IL-4, INF- ~ were detected by enzyme linked immunosorbent assay （ELISA）. The respiratory resistance and lung compliance of rats were measured by multichannel physiological signal collection processing system. Results Compared to control group, the serum levels of LTC4, TNFa ,ECP,IgE,IL-4 in model group were elevated significantly （P〈0.01 or P〈0.05）. Compared to control group, the respiratory resistance in model group was elevated （P〈0.01） and the lung compliance was reduced signifi- cantly （P〈0.01）. Conclusion The animal model of bronchiolitis was successfully established by infection of respiratory syncytial virus in rats.