胎儿5种器官凝血因子Ⅷ:C、Ⅸ:C和vWF:Ag测定的初步观察

Preliminary Observation of the Levels of Coagulation Factors ⅦC,ⅨC and vWFAg Determined in Five Organs of Fetus

为了确定凝血因子Ⅶ:C、Ⅸ:C 和 vWF:Ag 在胎儿期的发生部位,本文就用水囊引产法获得胎龄18～23周的胎儿,分别测定其肾、肝、脾、骨髓和胸腺等5种脏器中凝血因子Ⅷ:C、Ⅸ:C 及 vWF:Ag 的水平。并比较了肾组织培养前后凝血因子水平的变化。结果表明,肾脏组织凝血因子Ⅷ:C、Ⅸ:C 的水平明显高于其他组织。随着培养后细胞数的增殖,Ⅷ:C 和Ⅸ:C 的水平也有了提高。因此,初步认定胎儿肾脏组织可能是合成或贮存凝血因子Ⅷ:C 和Ⅸ:C 的器官之一。

In order to locate the generation site of coagulation factors ⅧC,ⅨC and vWFAg in fetus,the levels of coagulation factors ⅧC,ⅨC and vW- FAg had been determined in kidneys, livers,spleens,bone marrows and thymuses of the 18-23 weeks fetuses, got by induction with water bag. And,the changes in levels of these factors were compared before and after culture of the kidney issues. The results showed that the levels of coagulation factors ⅧC,ⅨC and vWFAg in kidney were significantly higher than those in other organs. The levels of coagulation factors ⅧC and ⅨC were raised in the process of cellular proliferation after culture. And therefore,one can come to the preliminary conclusion that the fetus kidney may be one of the organs that can synthesize or store the coagulation factors ⅧC and ⅨC