期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

XMRV在我国部分人群中的初步调查

Preliminary Investigation of XMRV in Some Populations in China
下载PDF
导出
摘要 目的检测XMRV是否存在于我国健康献血者和肿瘤患者体内。方法采用巢式反转录-聚合酶链反应方法,检测243例健康献血者和36例肿瘤患者的血液样本中的XMRV RNA,分析XMRV的感染情况。结果在所检测的279例血液样本中,XMRV RNA均呈阴性,各标本对应的内参引物三磷酸甘油醛脱氢酶基因(hGAPDH)均呈阳性。结论在所检测的样本中,没有发现XMRV的感染,需要进一步扩大样本量,探讨XMRV在中国的感染状况。 Objective To investigate the infection status of XMRV in healthy blood donors and cancer patients in China.Methods XMRV RNA was detected in 279 samples collected from 36 cancer patients and 243 blood donors by using a nested RT-PCR assay,and XMRV infection status was analyzed.Results XMRV RNA presented negative in all of the 279 samples tested.The internal reference primer glyceraldehyde phosphate dehydrogenase gene（hGAPDH）of each specimen was positive.Conclusion The presence of XMRV infection was not demonstrated in the tested samples.Expanded sample size is needed for further exploration of XMRV infection status in China.
作者 张莎娜 陆颖 王海平 王全立
机构地区 中国人民解放军总医院 军事医学科学院附属医院输血科
出处 《医学综述》 2012年第8期1240-1242,共3页 Medical Recapitulate
关键词 异嗜性小鼠白血病病毒相关病毒 健康献血者 肿瘤患者 巢式反转录-聚合酶链反应 Xenotropic MuLV-related virus Healthy blood donors Cancer patients Nested RT-PCR
分类号 R457.1 [医药卫生—治疗学]
  • 相关文献

参考文献10

  • 1Rodriguez JJ,Goff SP.Xenotropic murine leukemia virus-related vi-rus establishes an efficient spreading infection and exhibits en-hanced transcriptional activity in prostate carcinoma cells[J].JVirol,2010,84(5):2556-2562.
  • 2Silverman RH,Nguyen C,Weight CJ,et al.The human retrovirusXMRV in prostate cancer and chronic fatigue syndrome[J].NatRev Urol,2010,7(7):392-402.
  • 3Arnold RS,Makarova NV,Osunkoya AO,et al.XMRV infection inpatients with prostate cancer:novel serologic assay and correlationwith PCR and FISH[J].Urology,2010,75(4):755-761.
  • 4Schlaberg R,Choe DJ,Brown KR,et al.XMRV is present in malig-nant prostatic epithelium and is associated with prostate cancer,es-pecially high-grade tumors[J].Proc Natl Acad Sci USA,2009,106(38):16351-16356.
  • 5Sfanos KS,Sauvageot J,Fedor HL,et al.A molecular analysis ofprokaryotic and viral DNA sequences in prostate tissue from pa-tients with prostate cancer indicates the presence of multiple anddiverse microorganisms[J].Prostate,2008,68(3):306-320.
  • 6Knouf EC,Metzger MJ,Mitchell PS,et al.Multiple integrated cop-ies and high-level production of the human retrovirus XMRV(xeno-tropic murine leukemia virus-related virus)from 22Rv1 prostatecarcinoma cells[J].J Virol,2009,83(14):7353-7356.
  • 7Hohn O,Krause H,Barbarotto P,et al.Lack of evidence for xeno-tropic murine leukemia virus-related virus(XMRV)in Germanprostate cancer patients[J].Retrovirology,2009,6:92.
  • 8Lombardi VC,Ruscetti FW,Das Gupta J,et al.Detection of an in-fectious retrovirus,XMRV,in blood cells of patients with chronicfatigue syndrome[J].Science,2009,326(5952):585-589.
  • 9Dong BH,Silverman RH,Kandel ES.A Natural human retrovirusefficiently complements vectors based on murine leukemia virus[J].PLoS One,2008,3(9):e3144.
  • 10Urisman A,Molinaro RJ,Fischer N,et al.Identification of a novelGammaretrovirus in prostate tumors of patients homozygous forR462Q RNASEL variant[J].PLoS Pathog,2006,2(3):e25.
医学综述
2012年 第8期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部