增强化学发光法检测基质金属蛋白酶-11鉴定月经血

Detection of Matrix Metalloproteinase-11 in Menstrual Blood by Enhanced Chemiluminescence Method

目的探讨增强化学发光法检测人基质金属蛋白酶-11(matrix metalloproteinase-11,MMP-11)鉴定月经血的法医学应用价值。方法运用增强化学发光法检测月经血痕、阴道拭子、外周血痕、唾液斑、尿液斑和精液斑中的MMP-11,考察MMP-11的组织特异性、稳定性及检测方法的灵敏度。结果月经血痕中MMP-11蛋白的检测阳性率为89.47%,阴道拭子、外周血痕、唾液斑、尿液斑和精液斑中均未检出MMP-11。当上样量为25μL时,蛋白质量浓度为1.329μg/μL,可检出MMP-11。4℃放置20个月的MMP-11阳性的月经血痕,检测阳性率为89.58%。结论用增强化学发光法检测MMP-11蛋白的方法灵敏度高、特异性好,能用于月经血与外周血、阴道液等常见斑痕的鉴别。

Objective To explore the forensic application value of detection of matrix metalloproteinase-11(MMP-11) in menstrual blood by enhanced chemiluminescence method.Methods Menstrual blood,vaginal swab,peripheral blood,saliva stain,urine stain and semen stain were collected to detect whether or not there were MMP-11 using enhanced chemiluminescence method.The specificity and reliability of the MMP-11 assay along with its sensitivity were evaluated.Results The positive detection rate of MMP-11 in menstrual blood was 89.47%,whereas no MMP-11 was found in vaginal swab,peripheral blood,saliva stain,urine stain and semen stain.When 25 μL sample was added,the mass concentration of protein was 1.329 μg/μL,then MMP-11 could be detected.A positive detection rate of 89.58% was observed in MMP-11 positive menstrual blood samples after stored at 4 ℃ for 20 months.Conclusion Enhanced chemiluminescence method is sensitive and specific for detecting MMP-11,and can be applied to distinguish menstrual blood from common stain such as peripheral blood,vaginal fluid.