甘蔗糖蜜发酵产黄原胶发酵条件的优化研究

Optimization of the fermentation conditions of Xamthan Gum by using sugar-cane molasses

实验以甘蔗糖蜜为碳源发酵生产黄原胶,通过单因素实验和正交实验设计,确定优化的培养条件为:糖蜜酸化水解液糖度12°Bx,蛋白胨浓度8g/L,培养时96h,发酵温度28℃,接种量10%,pH7.5,在优化的培养条件下黄原胶的产量可达35.28g/L,是初始培养条件产量22.14g/L的1.59倍。

Xamthan gum was produced by using sugar-cane molasses,after the single factor and orthogonal design experiments,the optimal combinations were confirmed as followings：the content of sugar-cane molasses was 12°Bx,peptone was 8g/L,the inoculating content was 10%,initial pH was 7.5,and shaking temperature was 28℃,inoculating time was 96h.Under the optimized conditions,the Xamthan gum content was 35.28g/L,159% higher than the initial fermentation conditions.