毛细管气相色谱法测定肉制品中脱氢乙酸含量

Determination of Dehydroacetic Acid in Meat Products by Capillary GC

建立肉制品中脱氢乙酸含量的毛细管气相色谱测定方法。该法采用WBI进样口,毛细管色谱柱(弱极性)DB-5(30m×0.53mm,0.5μm),FID检测器进行检测。进样口温度:250℃;载气流速:10mL/min;程序升温:初始80℃,以10℃/min升至200℃,保持5min;检测器温度:250℃,样品用硫酸溶液酸化无水乙醇定容后直接进样测定。方法简单、快速、重现性好,平均回收率为97.5%,RSD为1.16%,线性范围为10～200μg/mL。

A method has been established for the determination of dehydroacetic acid in meat products by capillary GC with FID detection.The chromatographic separation was achieved on a capillary GC column DB-5（weak polarity,30 m × 0.53 mm,0.5 μm）.A WBI injection port was used and the temperature was set at 250 ℃.The flow rate of carrier gas was 10 mL/min.The initial temperature was 80 ℃,increased to 200 ℃ at a rate of 10 ℃/min and then hold for 5 min.The detector temperature was set at 250 ℃.Samples were acidified with sulfuric acid and made up to the required volume with absolute ethanol before direct injection for GC analysis.The method was simple,fast and reproducible.The average spike recovery from blank sausage was 97.5% and the relative standard derivation was 1.16%（n =6）.The linear range was 10-200 μg/mL.