转移性癌细胞体外水解酶表达及影响因素的作用

Expression of Proteolytic Enzymes of Metastatic Tumor Cells and the Effects of Some Influencing Factors

目的 为阐明树突状细胞肉瘤 (DCS)细胞在体外培养条件下其水解酶表达的类型及一些影响因素的作用。方法 采用水解空斑法检测 10种特异性蛋白酶抑制剂、不同抗体及基质对 DCS细胞的体外蛋白水解作用的影响。结果 抑肽酶 (aprotinin)、乙二胺四乙酸钠 (EDTA- Na2 )、抑胃酶 ((pepstatin)可显著抑制 DCS细胞的蛋白水解作用。抗泛素抗体、抗 DCS抗体对 DCS的蛋白水解作用无明显影响。在纤粘连蛋白 (FN)、层粘连蛋白 (L N)基质上 DCS细胞铺展良好 ,蛋白水解作用不明显。结论 本实验条件下 ,DCS细胞可表达丝氨酸蛋白酶、金属蛋白酶(非基质水解酶 )、天门冬氨酸蛋白酶的活性。抗泛素抗体、抗 DCS抗体对 DCS细胞的蛋白水解作用无明显影响。不同基质上

Objective To elucidate the types of proteolytic enzymes the dendritic cell sarcoma (DCS) cell expressed in vitro and the effects of modulating factors. Methods The proteolytic spots of DCS cells were examined, with ten different proteinase inhibitors, various antibodies and matrix. Results Aprotinin, EDTANa 2 and pepstatin could inhibit the proteolysis of DCS cells respectively. Antiubiquitin antibody and antiDCS antibody (1∶200~1∶100) showed no obvious inhibitory effects. DCS cells spread out fairly well but with no proteolysis. Conclusions Under the conditions of our experiments the DCS cells had serine proteinase, mettaloproteinase and aspartate proteinase activities. Antiubiquitin antibodies and antiDCS antibodies showed no obvious influence on the proteolysis of DCS cells. DCS cells displayed different proteolysis status on different matrix.