谷氨酰胺添加水平对热应激肉鸡小肠粘膜上皮内淋巴细胞数量的影响

Effects of Added Glutamine level on numbers of intraepithelial lymphocyte of small intestinal mucosal in Broilers Suffered from Heat Stress

研究了添加不同水平谷氨酰胺对热应激肉鸡小肠黏膜上皮内淋巴细胞数量的影响。选用1日龄科宝-500肉鸡240只,随机分为6个处理,每处理4个重复,每重复10只,Ⅰ组饲喂玉米-豆粕型基础日粮,Ⅱ、Ⅲ、Ⅳ、Ⅴ和Ⅵ组分别在基础日粮中添加0.4%、0.8%、1.2%、1.6%和2.0%的谷氨酰胺,试验时间15～42日龄,共28d。试验期间从每天早上0900到下午1700温度维持在35±1℃8h,下午1700至次日早上0900温度维持在30±1℃,鸡舍相对湿度控制在70%～80%。试验测定了热应激条件下21、28、35、42日龄肉鸡十二指肠、空肠、回肠粘膜上皮内淋巴细胞的数量。结果表明:日粮中添加谷氨酰胺显著提高了21d、28d、35d、42d热应激肉鸡十二指肠、空肠、回肠粘膜上皮内淋巴细胞的数量(P<0.05)。因此,在基础日粮中添加一定水平的谷氨酰胺可改善热应激肉鸡的肠道免疫性能。

The experiment was conducted to study the effects of Glutamine（Gln） on numbers of intraepithelial lymphocyte in broilers suffered from heat stress.Two hundred and forty Kebao-500 broilers at the age of 1 d were randomly selected and divided into six treatments with 4 replicates of 10 chicks in each replicate.Glutamine was supplemented at the level of 0%,0.4%,0.8%,1.2%,1.6% and 2.0% in six treatment basal diets respectively.The experiment lasted for four weeks.During the experiment period,the temperature maintained at 35±1 ℃ 8 h from 0900 AM to 1700 PM and maintained at 30±1 ℃ from 1700 PM to 0900AM of the next day.The relative humidity of the chicken house was controlled from 70% to 80%.The effects of Gln on numbers of intraepithelial lymphocyte of duodenum,jejunum,ileum in heat stressed broilers were studied.The results showed that,at the age of 21、28、35、42 d,supplemental Gln could enhance significantly on numbers of intraepithelial lymphocyte of duodenum,jejunum,ileum in heat stressed（P0.05）.Add a certain level of Gln in the basal diet can improve immunity capacity of heat stress broilers.