慢性肾小球肾炎尿毒症期血小板活化因子及肿瘤坏死因子α检测及临床意义

The detection and clinical significance of platelet-activated factor(PAF)and tumor necrosis factor-α(TNF-α) in the patients with uremia of chronic glomerulonephritis.

目的 探讨慢性肾小球肾炎尿毒症期患者血小板活化因子 (PAF)及肿瘤坏死因子α(TNF α)变化及意义。方法 采用生物活性法及双抗体酶联免疫吸附法 ,测定 14例慢性肾小球肾炎尿毒症期患者血浆PAF及血清TNF α水平。结果 慢性肾小球肾炎尿毒症期患者PAF及TNF α水平较正常对照组明显增高 ,差异有显著性 (P <0 .0 1) ,患者PAF及TNF α含量与尿素氮肌酐含量无明显相关性。结论 PAF与TNF α不仅参与了肾小球肾炎的发生 ,也可能持续参与了该病炎症的发展。二者之间的协同作用 ,加重了肾组织的广泛损伤 ,并影响到肾功能 ,PAF及TNF

Objective To value the clinical significance of serum Levels of platelet activated factor(PAF)and tumor necrosis factor α(TNF α) in the patients with uremia of chronic glomerulonephritis.Methods The serum changes of PAF and TNF α in the 14 cases of uremia of chronic glomerulonephritis were determined using the methods of bioactivity and ELISA.Results The serum PAF and TNF α levels of uremia,compared with the control group,were markedly increased(P<0.01).There was no correlation between PAF,TNF α and urea nitrogen creatinine levels.Conclusions Our study suggested that serum PAF and TNF α were involved in the pathogenesis of chronic glomerulonephritis.The relationship between PAF,TNF α levels and renal function could be investigated further.