协同刺激分子CD28/CTLA-4,B7在实验性变态反应性神经炎发病中的动态变化

Dynamic Changes of Costimulatory Molecules CD28/CTLA-4,B7 in Experimental Allergic Neuritis

目的探讨CD2 8/CTLA 4,B7在小鼠实验性变态反应言神经炎 (EAN )中的变化规律及对发病的影响。方法于小鼠双后肢足垫皮下注射兔坐骨神经匀浆 ,建立EAN模型 ,用免疫组化观察外周淋巴细胞CD2 8,CTLA 4,B7 1和B7 2蛋白的表达 ;用RT PCR检测外周淋巴细胞B7 1和B7 2mRNA的表达。结果B7 1蛋白和mR NA及CTLA 4蛋白从潜伏期至发病高峰呈递增趋势 ,而B7 2蛋白和mRNA及CD2 8蛋白从潜伏期至发病初期明显增高 ,至发病高峰又下降。结论CD2 8/CTLA 4,B7与EAN的发病密切相关。B7 2可能与EAN的始发有关 ,而B7 1与EAN的进展及维持关系更密切。

ObjectiveTo investigate the changing rules of CD28,CTLA 4,B7 1 and B7 2 as well as their effects on the onset of disease in experimental allergic neuritis(EAN).MethodsEAN models were established on mice by subcutaneous injection of homogenate prepared from rabbit sciatic nerves.The expression of CD28,CTLA 4,B7 1 and B7 2 proteins in lymphocytes of peripheral blood was examined with immunohistochemistry,and that of B7 1 and B7 2 mRNA with RT PCR dynamically on day 14(incubation period),day 18(primary period)and day 22(peak of disease)after mice immunization.ResultsExpression of B7 1 protein/mRNA and CTLA 4 protein increased progressively from incubation period to the peak of disease,whereas that of B7 2 protein/mRNA and CD28 protein increased markedly from incubation period to the early stage of disease,then decreased during the peak of EAN.ConclusionsThe increased expression of CD28/CTLA 4,B7 is closely related with the occurrence of EAN,while B7 2 is considered to be involved in the initiation of the disease and B7 1 in its progress and persistence.