正常雏鸡耳蜗中谷氨酸免疫反应分布

The localization of glutamate in the chicken cochlea

目的 :证实谷氨酸在耳蜗毛细胞 -听神经传入过程中的递质作用。方法 :用半薄切片单克隆抗体免疫组织化学技术 ,研究正常雏鸡基底乳头及螺旋神经节的谷氨酸免疫反应 ( Glutamate Imm unoreactivity,Glu- IR)。结果 :高、矮毛细胞胞浆均呈 Glu- IR阳性 ,毛细胞下神经纤维着色较淡 ,支持细胞反应阴性 ;高毛细胞反应较矮毛细胞反应强 ;螺旋神经节细胞呈圆形或椭圆形 ,胞浆 Glu- IR阳性 ,胞核不着色。结论 :Glu可能作为小鸡耳蜗传入神经递质 (或调质 )

Objective:To prove that glutamate plays a role as transmitter in cochlear herrus acusticus introduction.Method:Glutamate immunoreasctivity (Glu IR) was investigated in the cytoplasm of the hair cells ganglion cells, and nerve fibers in normal chickens by means of monoclonal antibody immunocheming.Result:The tall hair cells reacted more strongly than the short hair cells.The supporting cells showed no positive stain.It was suggested that glutamate may be the transmitter or modulator in the chicken cochlea.Conclusion:Glumate may act as fransmitter in cochlear introduction.