内南五味子木脂素戈米辛J对肝线粒体膜脂质过氧化和超氧阴离子自由基的作用

Effects of lignan gomisin J from kadsura interior on liver mitochondria lipid peroxidation and on the superoxide anion radical

目的 研究来自内南五味子的戈米辛J对脂质过氧化的影响和清除超氧阴离子自由基 (O·2 )的能力。方法 采用离体大鼠肝线粒体膜的脂质过氧化模型和黄嘌呤氧化酶 鲁米诺化学发光法。结果 戈米辛J象VitE一样能剂量依赖性抑制Fe2 + VitC和ADP/NADPH所致的脂质过氧化 ;戈米辛J的IC50 分别为 5 75 ( 95 %可信限 :5 42～ 6 11)和0 95 ( 0 14～ 6 5 4) μmol·L-1。VitE的IC50 分别为 74 8( 30 2～ 185 3)和 6 5 1( 0 13～ 319) μmol·L-1。戈米辛J抑制Fe2 + VitC和ADP/NADPH所致的脂质过氧化的作用比VitE的作用分别强 13倍和 6 8倍。戈米辛J也剂量依赖地抑制黄嘌呤 黄嘌呤氧化酶 鲁米诺化学发光 ,其抑制发光强度 5 0 %的浓度 (IC50 )为 2 18 2 2 μmol·L-1。结论 戈米辛J具有抑制OH·诱导的脂质过氧化和清除O·2

AIM To study the influences of gomisin J on lipid peroxidation and the superoxide anion radical METHODS Two in vitro models of rat liver mitochondria membrane lipid peroxidation (LPO) and the xanthinexanthine oxidase reaction in Luminoldependent chemiluminoscence system (CL system) were used RESULTS Gomisin J as like Vit E could dosedependently inhibit Fe 2+ Vit C and ADP/NADPHinduced LPO with IC 50 (95% confidence limits) 575 (542～611) and 095 (014～654) μmol·L -1 , respectively Vit E's IC 50 values (95% confidence limits) were 748 (302～1853) and 651 (013～319) μmol·L -1 , respectively, The potency of gomisin J was 68 and 13 times than that of Vit E Gomisin J could inhibit the xanthinexanthine oxidase reaction with its dosedependency in Luminoldependent CL system Its IC 50 value was 2182 μmol·L -1 CONCLUSION Gomisin J could inhibit LOP induced by hydroxy free radical in rat liver mitochondria and scavenge the superoxide anion radical