山慈菇快速繁殖体系的建立

Establishment of Rapid Multiplication System for Iphigenia indica

以山慈菇的组织芽块外植体为试材,采用单因素变量法,通过对基本培养基、大量元素、单一激素、激素组合等因素的研究,探讨最适宜的山慈菇培养方案,建立山慈菇快速繁殖体系。结果表明:最适宜组织芽块外植体生长的培养基为1/4MS+0.1mg/L NAA+2.0mg/LBA+3%蔗糖+0.5%活性炭+0.55%琼脂,pH 5.8,培养45d,生长率为51.03%,增重率为52.49%。

Chosen buds piece of explant the Iphigenia indica as test material,by the methods of single factorial variable,including basic medium,the content of large element,single hormone,hormone combination and etc were studied,appropriate cultirate scheme were discussed,and rapid proliferation system of iphigenia indica were establishmented.The rensults showed that the culture medium where the tissue bud had the fastest growth was 1/4MS medium with 0.1 mg/L NAA,2.0 mg/L BA,3% sucrose,0.5% active carbon,0.55% agar.The growth rate and weighting rate was 51.03% and 52.49%.