多效唑预处理对红金银花直接培养成壮苗的影响

Effect of Pretreatment with PP_(333) on Culture Directly into Seedling to Lonicera japonica var.chinensis

用浓度为0(CK)、200、500mg/kg的多效唑分别对盆栽的红金银花提前1个月灌根处理,其后将经过多效唑处理的红金银花顶芽、带侧芽的茎段灭菌后,接在最适合顶芽、带侧芽的茎段诱导的培养基上进行培养,以期研究不同浓度的多效唑对盆栽红金银花壮苗的影响。结果表明:10～15d后,各处理的外植体均萌动,愈伤组织逐渐出现;35～45d后,叶、茎抽生;60～70d后,用多效唑预处理抽生的枝条比对照多,节间较短,叶色浓绿,叶面积较对照小,各处理的组培苗长势差异显著,以200mg/kg处理所成苗茎叶粗壮、500mg/kg次之,对照最差。

With a concentration of 0,200,500 mg/kg respectively of PP333 on potted plants of Lonicera japonica var.chinensis one month in advance were treated to the root,and then the bud and stem segment with axillary bud after PP333 treatment were connected to culture to the most suitable culture medium after eradicating bacterium.The effect of pretreatment with PP333 on culture directly into seedling to Lonicera japonica var.chinensis were studied.The results showed that all the explants were germinated,injury organization gradually appeared after 10~15 d;the leaves,and stems were normal pumping after 35~45 d;the branches with PP333 pretreatment were sprouting more than CK,the internodes were short,the leaves were dark green,the leaf area was small than CK after 60~70 d.The growth of the tissue culture seedlings of all the treatments were different significantly,the stems and leaves of the culture seedlings with 200 mg/kg treatment were stout,and 500 mg/kg followed by,CK was the worst.