菊花品种表型性状与SRAP分子标记的关联分析

The Association Analysis of Phenotypic Traits with SRAP Markers in Chrysanthemum

【目的】寻找与菊花重要园艺性状相关联的分子标记,为菊花复杂数量性状的研究以及分子标记辅助育种奠定遗传学基础。【方法】利用筛选出的19对SRAP引物组合对58个典型大菊品种进行多位点扫描分析。在对供试材料进行群体结构分析的基础上,利用TASSEL软件,对获得的分子标记与这些品种的18个重要表型性状进行关联分析。【结果】群体遗传结构分析将58个大菊品种划分为5个亚群结构:平瓣类、管瓣类、畸瓣类、桂瓣类和日本品种亚群;通过关联分析,发现有6个标记位点与5个性状关联(P<0.01),其中与花部性状(花梗粗度、花瓣宽度、筒状小花数量)相关位点共5个,与茎部(茎粗度)相关位点1个,与叶部性状(叶厚度)相关位点1个,各位点对表型变异的解释率在0.0738—0.4791。【结论】利用SRAP标记可有效地对菊花进行群体结构的判断和划分。关联分析能够有效地找到与菊花表型性状关联的SRAP标记,用于分子标记辅助育种。

【Objective】 In order to provide a genetic basis for studies on complex quantitive traits and for molecular assisted breeding of chrysanthemum,the SRAP markers associated with important chrysanthemum horticulture traits were screened.【Method】 The genomic regions with selection sweep were detected through scanning 58 representative chrysanthemum cultivars using 19 SRAP markers.Population structure was firstly analyzed,then association analysis between SRAP markers and 18 important phenotypic traits were performed using TASSEL GLM.【Result】 Genetic structure analysis showed that the selected cultivar population was composed of 5 subpopulations,namely flat type subgroup,tube type subgroup,irregular type subgroup,anemone type subgroup and Japanese subgroup.There were 6 SRAP loci associated with 5 quantitative characters（P0.01）,among which 3 flower traits were associated with 5 loci,while 1 stem and 1 leaf traits were associated with 1 locus,respectively.The rate of explanation on the phenotype of related locus ranged from 0.0738 to 0.4791.【Conclusion】It is feasible to estimate and differentiate chrysanthemum population＇s structure effectively using SRAP markers,and the markers obtained in this study are promising in molecular assisted breeding.