MicroRNA-29b在卵巢上皮性癌中的表达及临床意义

Expression and clinical significance of microRNA-29b in the epithelian ovarian cancer

目的:检测MicroRNA-29b(miR-29b)在卵巢上皮性癌组织的表达,并分析miR-29b与卵巢上皮性癌临床病理特征之间的关系。方法:采用实时荧光定量聚合酶链反应(qRT-PCR)定量分析60例卵巢上皮性癌,15例卵巢良性肿瘤及15例正常卵巢组织中miR-29b的表达,并将检测结果与临床指标进行统计学分析。结果:(1)卵巢上皮性癌组织中miR-29b的表达量显著低于正常卵巢及卵巢良性肿瘤组织(P<0.05)。(2)miR-29b表达量与FIGO分期、淋巴结转移及腹水产生相关(P<0.05),与患者年龄、细胞分化、病理类型、残灶直径等临床病理参数无关(P>0.05)。Ⅲ～Ⅳ期和淋巴结转移组的表达量分别低于Ⅰ～Ⅱ期和无淋巴结转移组(P<0.05);腹水组miR-29b的表达量亦低于无腹水组(P<0.05)。(3)Kaplan-Meier法比较生存曲线表明,miR-29b表达量低者术后生存时间短(P<0.05);COX多因素生存分析表明,miR-29b低表达与患者生存时间短独立相关,低表达组的死亡危险度是高表达组的3.996倍。结论:miR-29b可能作为抑癌因子参与了卵巢上皮性癌的发生发展,对卵巢上皮性癌具有潜在的辅助诊断及预后评估意义。

Objective：To detect the miR-29b expression in the epithelial ovarian cancer,and analyze the correlation of its expression with clinicopathological features.Methods：real-time PCR was employed to quantify the miR-29b expression in 90 ovarian specimens（including 15 normal,15 benign and 60 malignant）.The relevance between the miR-29b expression and the pathoclinical characteristics was assessed with statistics analysis.Results：（1）The expression of miR-29b was significantly down-regulated in patients with ovarian carcinoma compared to those with ovarian benign tumors and normal controls.（2）miR-29b expression was significantly associated with FIGO stage,lymph node metastasis and presence of hydroperitoneum（P0.05）,but not with ages,grades of cytology,differentiation,histological types,and sizes of residual tumors in epithelial ovarian cancer（P0.05）.In stage Ⅲ~Ⅳ and in the group with lymph node metastasis miR-29b were significantly down-regulated compared to those in stageⅠ~Ⅱ and in the group without lymph node metastasis respectively（P0.05）.Hydroperitoneum was associated with low expression of miR-29b（P0.05）.（3）Kaplan-Meier method showed that patients survived shorter when ovarian cancer tissues showed lower expression of miR-29b（P0.05）;COX proportional risk model analysis indicated that lower expression of miR-29b was independently related to survival time,and the risk of mortality with lower expression of miR-29b was 3.996 times as large as that with high expression.Conclusions：miR-29b may participate the origination and progression of epithelial ovarian cancer as a tumor-suppressor.It may represent potential indicator in the auxiliary diagnosis and prognosis of epithelial ovarian cancer.