纳米SnSe_2空心球上乙酰胆碱酯酶的固定及辛硫磷传感器的构建

Immobilization of Acetylcholinesterase on SnSe2Hollow Spheres for Sensitive Detection of Phoxim

描述了一种将乙酰胆碱酯酶固定在SnSe2空心球上检测辛硫磷的简单方法,用水热法合成了SnSe2空心球,并用透射电镜对其表征.固定的乙酰胆碱酯酶能保持其生物学活性,催化乙酰胆碱为胆碱,胆碱被氧化产生可检测的信号.基于辛硫磷对乙酰胆碱酯酶活性有抑制作用这个机理,在理想条件下,这种传感器对辛硫磷检测的线性范围是0.008～56μg/mL,检测限为0.004μg/mL.这种新型的传感器有很好的稳定性和重现性.这项工作表明SnSe2空心球可以作为固定乙酰胆碱酯酶的理想载体并用于构建相应的传感器.

A simple method for immobilization of acetylcholinesterase（AChE） on SnSe2 hollow spheres for detection of phoxim was described.SnSe2 hollow spheres were prepared by hydrothermal methods and were characterized by TEM.The immobilized AChE retained its biological activity well and could catalyze the hydrolysis of acetylthiocholine to form thiocholine,which was then oxidized to produce detectable signal.Based on the inhibition toward the enzymatic activity of AChE by phoxim,under optimal conditions,the sensors could be used for the determination of phoxim ranging from 0.008 to 56 μg/mL with the detection limit of 0.004 μg/mL.The developed phoxim biosensors exhibited good stability and reproducibility.This work demonstrated that SnSe2 hollow sphere could be served as an ideal carrier for immobilization of AChE to fabricate corresponding biosensor.