舒肝宁注射液对模型大鼠肝硬化腹水的改善作用研究

Improvement Effect of Shuganning Injection on Model Rats with Ascites Due to Cirrhosis

目的:研究舒肝宁注射液对大鼠肝硬化腹水的改善作用。方法:以腹腔注射四氯化碳(CCl4)复制大鼠肝硬化模型。实验分为正常对照(等容生理盐水)、模型(等容生理盐水)和舒肝宁注射液高、低剂量(2、0.5mL·kg-1)组。测定体重、肝指数、腹水量,检测血清肝功能指标、肝纤维化指标、心钠肽(ANP)和血管紧张素Ⅱ(ANGⅡ)含量。结果:与模型组比较,舒肝宁高剂量组大鼠腹水量显著减少(P<0.05);舒肝宁高、低剂量组大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、γ-谷酰转肽酶(GGT)活性显著减弱(P<0.01或P<0.05);舒肝宁高剂量组大鼠血清玻璃酸HA、Ⅲ型前胶原(PCⅢ)、)、Ⅳ型胶原(ⅣC)、层粘连蛋白(LN)显著降低(P<0.05),ANP和ANGⅡ含量显著降低(P<0.05)。结论:舒肝宁注射液对大鼠肝硬化腹水具有治疗作用。

OBJECTIVE：To study the improvement effect of Shuganning injection on rats with ascities to cirrhosis.METHODS：Cirrhotic rat model was induced by intraperitoneal injection of CCl4.The rats were randomized into 4 groups,i.e.normal control group（constant volume normal saline）,model group（constant volume normal saline）,Shuganning injection high-dose and low-dose groups（2,0.5 mL·kg-1）.Body weight,liver index,ascities volume were determiedn,and serum liver function,biomarker for liver fibrosis,atrial natriuretic pepetide（ANP） and angiotensinⅡ（ANGⅡ） were measured.RESULTS：Compared with model group,ascities volume significantly reduced in Shuganning high-dose group（P0.05）;ALT,AST and GGT of Shuganning high-dose and low-dose groups were decreased significantly（P0.01 or P0.05）;HA,PCⅢ and ⅣC and the content of ANP ANGⅡ and LN in Shuganning high-dose group were decreased significantly（P0.05）.CONCLUSION：Shuganning injection has and therapeutic effect on rat with ascities due to cirrhosis.