HPLC法测定日本薯蓣中尿囊素的含量

Content Determination of Allantoin in Dioscorea japonica by HPLC

目的:建立测定日本薯蓣中尿囊素含量的方法,并比较日本薯蓣与山药中尿囊素的含量。方法:采用高效液相色谱法。色谱柱为Kromasil C18(250mm×4.6mm,5μm),流动相为甲醇-水(2:98),流速为0.8mL·min-1,检测波长为224nm,柱温为30℃。结果:尿囊素进样量在0.355～4.260μg范围内与峰面积积分值呈良好线性关系(r=0.9998);平均加样回收率为98.77%,RSD=1.49%(n=6)。日本薯蓣明显高于山药中尿囊素的含量。结论:该方法可作为日本薯蓣中尿囊素的含量测定方法。

OBJECTIVE：To establish a method for the content determination of allantoin in Dioscorea japonica,and to compare the content of allantoin in D.japonica and D.opposita.METHODS：HPLC was adopted.Kromasil C18（250 mm×4.6 mm,5 μm） column was used with methanol-water （2：98） as mobile phase at the flow rate of 0.8 mL·min-1.The detection wavelength was set at 224 nm,and the column temperature was at 30 ℃.RESULTS：The linear range of allantoin was 0.355～4.260 μg（r=0.999 8） with average recovery of 98.77%（RSD=1.49%,n=6）.The content of allantoin in D.japonica was higher than in D.opposita significantly.CONCLUSION：The method could be used for the content determination of allantoin in D.japonica.