5-FU对人结肠癌HCT116细胞摄取18F-FDG的影响

Inhibitory Effect of 5-FU in the Uptake of 18F-FDG on Human Colon Carcinoma Cell HCT 116

目的 探讨结肠癌HCT-116细胞对18F-FDG摄取率的影响因素,从而利用18F-FDG细胞摄取率的变化早期评价化疗的疗效.方法 依据实验结果依次改变实验条件中细胞浓度、反应时间、18F-FDG放射性活度、葡萄糖的浓度,测定结肠癌HCT-116细胞的18F-FDG细胞摄取率.在加入不同浓度5-FU前后,分别采用四甲基偶氮唑盐法（MTT）和细胞18F-FDG 摄取率测定细胞的增殖抑制率.结果 当细胞浓度1×106/瓶、加入18F-FDG放射性活度为3.7KBq,葡萄糖浓度为5.5mmol/ L,反应时间为100min,细胞摄取率为（43.93±3.54）%,加入终浓度10、20、30和50μg/mL 5-FU 100μL作用24h后,细胞摄取率分别为（32.47±5.43）%、（26.44±3.62）%、（22.26±3.63）%、（16.10±6.40）%;细胞摄取抑制率分别为 （25.81±5.64）%、（39.58±3.74）%、（49.31±5.16）%、（62.27±7.50）%,各组之间差异均有统计学意义,且随5-FU 剂量增加而下降,两者呈负相关（r=-0.879,P＜0.01）.给药24h后MTT实验细胞生长抑制率与细胞摄取抑制率呈正相关 （r=0.831,P＜0.01）.结论 5-FU作用24h后结肠癌HCT-116细胞18F-FDG摄取率下降,18F-FDG摄取抑制率升高,可以通过18F-FDG细胞摄取抑制率早期评价5-FU对结肠癌的杀伤作用.

Objective To establish a stable method to detect the 18F-Fluoro-deoxyglucose（18F-FDG） uptake in HCT-116 cell,and using 18F-FDG uptake inhibition rate to evaluation early response of chemotherapy on colon cancer cell HCT-116.Methods Uptake rate of 18F-FDG in HCTll6 were determined when the experimental conditions were changed in turn,including cell concentration,reaction time,18F-FDG radioactive activity and glucose concentrations incubation in 37℃.Before and after giving different concentrations 5-FU,the MTT method was adopted to measure cell proliferation inhibition rate and 18F-FDG uptake rates were also measured. Results Uptake rate of 18F-FDG was （43.93 ± 3.54）% at the condition of 1 × 106 cells,18F-FDG radioactive activity of 3.7KBq,glucose concentration of 5.5mmol/L and 100min incubation at 37℃.The uptake rates and uptake inhibition rates of 18F-FDG were（100min incubation at 37℃）（32.47 ± 5.43）%,（26.44 ± 3.62）%,（22.26 ± 3.63）%,（16.10 ± 6.40）% and （25.81 ± 5.64）%,（39.58 ± 3.74）%,（49.31 ± 5.16）%,（62.27 ± 7.50）% at the condition of 1 × 105 cells,3.TKBq 18F-FDG,5.5mmol/L glucose at 24h after the administration of 10,20,30 and 50μg/mL 5-FU,respectively.The 18F-FDG uptake was negatively correlated with the dose of 5-FU（r=-0.879,P 〈 0.01）.The uptake inhibition rate was positively correlated with the proliferation inhibition（r=0.831,P 〈 0.01）.Conclusion The absorbing efficiency of 18F-FDG on HCT116 decreased at 24h after administration of 5-FU.18F-FDG uptake is expected to reflect the inhibitory effect of 5-FU on colon cancer as early as 24h.