2型糖尿病大鼠不同药物治疗后内皮脂肪酶的表达变化

Analysis of endothelial lipase expression after different drugs treatment in type 2 diabetic rat

目的:检测2型糖尿病大鼠血浆中内皮脂肪酶(endothelial lipase,EL)水平,以及经不同降糖药物治疗后EL水平变化。探讨EL与2型糖尿病的相关性,评价不同降糖药物对EL水平的影响。方法:成年雄性SD大鼠60只,随机抽取15只作为正常对照组(CTL组),另外45只采取高脂高糖饲料喂养+链脲佐菌素诱导的方法制备2型糖尿病大鼠模型,而后将成模大鼠39只随机分为3组:2型糖尿病组(n=13,DM组)、胰岛素治疗组(n=13,IDM组)和格列齐特治疗组(n=13,GDM组),分别于1、9和13周末测体重、空腹血糖、空腹胰岛素水平、胰岛素敏感指数、甘油三酯、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、C-反应蛋白和EL。结果:13周末测各组血糖,DM组较CTL组、GDM组、IDM组升高(P<0.05)。其中IDM组、GDM组与CTL组无明显差异(P>0.05)。13周末测各组血浆EL水平,DM组EL表达较CTL组和IDM组明显升高(P<0.05),与GDM组无明显差异(P>0.05)。IDM组EL表达较DM组明显降低(P<0.05),与CTL组无明显差异(P>0.05)。结论:2型糖尿病大鼠中EL表达明显增高,经胰岛素治疗和格列齐特治疗后EL的表达并不一致。胰岛素在降低血糖的同时降低EL水平,而格列齐特降低血糖并不降低EL水平。这种不一致可能是由于胰岛素与格列齐特对EL的调节作用不同引起的。

Objective To detect the plasma endothelial lipase（EL） levels and the changes of EL levels after different hypoglycemic drugs therapy in type 2 diabetes rat.To explore the correlation of EL and type 2 diabetes mellitus,and evaluate the effect of different hypoglycemic drugs on the level of EL.Methods The type 2 diabetic rat model was set up by high-fat and high-sugar diet plus streptozotocin.Sixty adult male SD white rats were divided into two groups at random：the healthy control group（n = 15,HTL） and high-fat and high-sugar diet plus STZ-induced diabetic rats（n = 45）,then the model rats were randomly divided into three groups：the diabetic group（n = 13,DM）,insulin-treated DM group（n = 13,IDM） and gliclazide-treated DM group（n = 13,GDM）.Body weight,fasting blood glucose,fasting insulin levels,insulin sensitivity index,triglycerides,total cholesterol,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,C-reactive protein,EL were respectively measured at 1,9 and 13 weeks.Results At the end of 13 weeks,blood glucose in each group was measured.Compared with the HTL group,GDM group and IDM group,blood glucose in DM group increased obviously（P 0.05）,while there was no significant difference between IDM group,GDM group and HTL group（P 0.05）.At the end of 13 weeks,plasma EL levels were measured.Compared with the HTL group and IDM group,EL expression in DM group was significantly higher（P 0.05）,and there was no significant difference between IDM and GDM group（P 0.05）.EL expression in IDM group was significantly lower Compared with DM group（P 0.05）,but no significant difference was observed with HTL group（P 0.05）.Conclusions The expression of EL was significantly increased in type 2 diabetic rats.By insulin and gliclazide therapy,EL expression was not consistent.Insulin lowered blood glucose levels but also reduced EL,and gliclazide lowered blood glucose levels but did not reduce EL.The phenomena may be due to different regulation mechanisms of insulin and gliclazide for EL metabolism.