军团菌感染与2个细胞因子基因多态性关系

Relationship between gene polymorphisms of TNF-α and IL-1β 2 gene locus and Legionella infection

目的探索肿瘤坏死因子-α(TNF-α)-308位点、白介素-1β(IL-1β)-511位点基因多态性与军团菌感染的关系。方法将32例军团菌感染者作为病例组,随机抽取100例军团菌检测阴性的肺部感染者为患者对照组,同期收集70例健康体检者为健康人对照组,采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术检测3组TNF-α基因-308位点、IL-1β基因-511位点的单核苷酸多态性。结果病例组TNF-α-308基因型GG、GA、AA分别占75%、25%、0%,患者对照组GG、GA、AA基因型分别占87%、13%、0%,健康人对照组GG、GA、AA基因型分别占68.6%、31.4%、0%;病例组与患者对照组比较、病例组与健康人对照组比较,TNF-α-308位点基因型和等位基因频率差异无统计学意义(χ2=0.437、0.362,P=0.509、0.548;χ2=2.609、2.384,P=0.106、0.123);病例组IL-1β-511基因型CC、CT、TT分别占:0%、53.1%、46.9%,患者对照组CC、CT、TT基因型分别占3.4%、48.3%、48.3%,健康人对照组CC、CT、TT基因型分别占10%、51.4%、38.6%,3组比较,IL-1β-511位点基因型和等位基因频率差异无统计学意义(χ2=4.466,P=0.334;χ2=2.420,P=0.298)。结论 TNF-α-308位点、IL-1β-511位点的基因多态性与军团菌感染无关,TNF-α基因和IL-1β基因不是军团菌感染的易感基因。

Objective To explore the the relationship between gene polymorphisms of TNF-α-308 G/ A and IL-1β-511 C /T and Legionella infection. Methods Thirty-two individuals infected with Legionella were selected as case group and 100 other pulmonary disease patients with negative Legionella test were selected as and control group by randomized sampling,and 70 healthy individuals having health examination in the same period were chosen as another control group.The tumor necrosis factor-α（TNF-α）-G308A and interlenki-1β（IL-1β）-C511T gene polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）. Results In case group,the frequency of GG,GA,and AA of TNF-α-308 was 75%,25%,and 0% in the disease control group,the frequency of GG,GA,and AA and 87%,13%,and 0%;in the healthy control group,the frequency of GG,GA,and AA was 68.6%,31.4%,and 0%.There was no statistical difference for the TNF-α-G308A genotype and allele frequencies between case group and control groups（χ2=0.437,0.362,P=0.509,0.548;χ2=2.609,2.384,P=0.106,0.123）.In case group,the frequency of CC,CT,and TT of IL-1β-511 was 0%,53.1%,and 46.9%;in the disease control group,the frequency of CC,CT,and TT was 3.4%,48.3%,and 48.3%;in the healthy control group,the frequency of CC,CT,and TT was 10%,51.4%,and 38.6%.There was no statistical difference for the IL-1β-C511T genotype and allele frequencies between three groups（χ2=4.466,2.420,P=0.334,0.298）. Conclusion The gene polymorphisms of TNF-α-G308A and IL-1β-C511T was not associated with Legionella infection.TNF-α gene and IL-1β gene may be not the predisposing genes of Legionella infection.