霍乱弧菌04-5a糖发酵激活蛋白编码基因分析

Clonging and sequencing of sugar fermentation stimulation protein gene from Vibrio cholerae 04-5a

目的比较霍乱弧菌04-5a株糖发酵激活蛋白编码基因与霍乱流行株与非流行株相关序列的差异,明确其基因型。方法采用基因克隆测序方法,对霍乱弧菌04-5a株糖发酵激活蛋白编码基因进行序列分析,并与相关序列进行比较。结果霍乱弧菌04-5a株糖发酵激活蛋白编码基因由771个碱基对组成,编码258个氨基酸的多肽,流行株和非流行株相关序列比较发现,04-5a株与流行株两者基因完全相同,与非流行株在334、622和666位核苷酸碱基的具有差异,第666位碱基的差异导致了氨基酸的不同,在非流行株与流行株分别为丙氨酸与苏氨酸。结论霍乱弧菌04-5a株糖发酵激活蛋白编码基因序列符合流行株基因特征,在非流行株与流行株第666位碱基差异可能与其致病性及流行有关,值得深入研究。

Objective To investigate the differences in the sequences of sugar fermentation stimulation protein genes between Vibro cholerae 04-5a and epidemic and nonepidemic V.cholerae strains. Methods Cloning and sequencing methods were used to analyze the sequence of sugar fermentation stimulation protein genes from V.cholerae 04-5a and to compare the sequences with related gene sequences. Results The sequence of sugar fermentation stimulation protein genes from V.cholerae 04-5a is a 771bp gene,encoding 258 amino acid polypeptide,and is identical to the ones from the epidemic strain.According to the sequence of sugar fermentation stimulation protein gene,the V.cholerae 04-5a has mutations at 334th,622th and 666th point compared with the nonepidemic strain,and is identical to the epidemic strain.The difference of the 666th point mutation led to the changs of the deduced amino acid level. Conclusion The sequence of sugar fermentation stimulation protein gene from V.cholerae 04-5a is identical to that of from the epidemic strains and the related pathogenic mechanism and epidemiology need to be further studied.