宁波市水痘-带状疱疹病毒流行株基因分型

Genotype of prevalent varicella-zoster virus in Ningbo city

目的分析浙江省宁波市2009—2010年水痘的流行病学特征,以及水痘-带状疱疹毒流行株基因分型特征,为预防控制水痘提供依据。方法根据法定传染病报告系统、宁波市全国突发公共卫生事件报告管理系统的水痘发病资料,收集数据并统计分析;同时对部分临床诊断水痘病例采集疱疹液标本,采用MRC-5细胞进行VZV分离,并提取脱氧核糖核酸进行聚合酶链反应,并对扩增产物测序,分析其基因型别。结果宁波市2009、2010年水痘报告发病率分别为23.33/10万和26.24/10万,各县(市)区均有病例报告,发病年龄组以7～12岁儿童发病率最高(245.21/10万),存在2个发病时间高峰,分别为5～6月份和11～12月份。15例患者水痘病毒核酸检测阳性,13例患者VZV为J型毒株,2例为M1型。结论宁波市水痘病例主要发生在冬春季节,主要以7～12岁及学龄儿童为主,J型毒株目前为本市流行主要毒株,但也有M1型毒株的局部流行。

Objective To examine the epidemiological characteristics of varicella and to detect the genotype of varicella-zoster virus for developing strategies of varicella prevention and control in Ningbo. Methods Graphs and appropriate statistics were used to describe the epidemiological characteristics of chickenpox cases extracted from the China Disease Prevention and Control Information System.Meanwhile herpes specimen were collected from clinically diagnosed cases and genotypes of varicella-zoster virus were determined with polymerase chain reaction（PCR） technique. Results The reported incidence of varicella in Ningbo was 23.33/100 000 in 2009 and 26.24/100 000 in 2010,respectively.Most of the patients were children aged 7-12 years and the epidemic peaks were in May,June,November and December.The genotypes of 15 DNA specimens were 13 J-type and 2 M1-type. Conclusion Varicella cases in Ningbo occurred mainly in winter and spring seasons and most of the cases were children aged 7 to 12 years.The predominant strain circulated in Ningbo is J-type,with M1-type strain isolated sporadically.