生物防护服穿透性噬菌体指标筛选

Selection of phage indexes for evaluating penetrative performance of biological protection materials

目的研究PhiX-174、f2、MS2 3种噬菌体的生物学性质,为生物防护服抗微生物悬浮液穿透性能评价选择合适的指示噬菌体。方法从噬菌体形态大小、繁殖能力、耐压性及其所形成噬菌斑的特征等方面,对PhiX-174、f2、MS2 3种噬菌体的生物学性质进行观察。结果 PhiX-174形成噬菌斑最大、形成时间最短,培养5 h后,噬菌斑直径可达2.57 mm,繁殖能力最强,4 h即可迅速增殖到1.67×1010 PFU/mL,最终可达1012 PFU/mL以上,3种噬菌体大小相当,直径均为25 nm左右,远小于人类血源性病毒和呼吸道病毒,均有一定耐压力,在1.75、3.5、7、14、20 kPa下作用5 min后,均未见噬菌体滴度明显减少。结论噬菌体PhiX-174最适于作为生物防护服尤其高危生物污染环境作业人员防护服抗病毒效果评价的指示病毒。

Objective To select a suitable phage indicator for evaluating the ability of biological protection materials against microbial suspension penetrative performance. Methods The morphous,the reproductive capacity,the ability to stand pressure and the plaque morphous of PhiX-174,f2,and MS2 were studied. Results PhiX-174 could not only form the biggest plaque in the shortest time,but also showed the most powerful reproductive capacity.Culturing for 5 hours,the diameter of the plaque formed by PhiX-174 was 2.57 mm.In 4 hours culturing,the number of PhiX-174 was 1.67×1010 plaque forming unit（PFU）/mL and exceeded 1012 PFU/mL in the last.The diameters of PhiX-174,f2,and MS2 were approximately about 25 nm,far smaller than human haematogenous virus and respirovirus.PhiX-174,f2,and MS2 could resist to a certain stress.When under high pressure（1.75,3.5,7,14,and 20 kPa/cm2） for 5 minutes,the titers of the phages showed no significant decrease. Conclusion PhiX-174 is the most suitable phage indicator to evaluate the anti-viral effect of biological protective clothing,especially the protective clothing for the people doing high-risk job in biological pollutant environment.