养分胁迫对水稻籽粒灌浆充实影响的蛋白质组学研究

Proteomics Analysis of Nutrient Deficiency Effect on Rice Grain Filling

以杂交早稻威优916为试验材料,采用水培方式栽培,生育后期设置养分胁迫和全营养液两种处理,从蛋白质组学角度研究后期持续的养分胁迫对水稻籽粒灌浆的影响,以期为水稻高产栽培提供科学依据。籽粒蛋白质经双向电泳分离后共获得了37个发生差异表达的蛋白质,经串联质谱分析(ESI-Q MS/MS),27个蛋白质功能得到鉴定,包括4个参与光合作用的蛋白质、13个与籽粒充实发育相关的蛋白质、9个逆境相关的蛋白质及1个呼吸代谢相关的蛋白质。分析结果表明,养分胁迫下,逆境相关蛋白质在籽粒灌浆的前中期大量上调表达,籽粒光合作用减弱,后期呼吸作用增强,籽粒的氮代谢受到显著影响,谷蛋白及胚蛋白表达相对滞后,蛋白质和脂肪合成减少,从而造成籽粒灌浆不足,产量降低。

In order to determine the effect of nutrient deficiency on rice grain filling and lay the scientific basis for high- yielding cultivation, the protein expression profiles in grains of early hybrid rice Weiyou 916 were analyzed by proteomics approach under nutrition deficiency and full nutrition application at late developmental stage. Two- dimensional electrophoresis gel analysis showed that 37 protein spots changed in abundance, 27 proteins involved photosynthesis （4 proteins）, grain development （13 proteins）, stress response （9 proteins） and respiration （1 protein） were identified by ESI-Q MS/MS. Under nutrient deficiency conditions, proteins associated with stress response upregulated in expression abundance at early and middle filling stage, photosynthesis decreased and respiration increased at later stage. The nitrogen metabolism was affected by nutrient deficiency, resulting in delayed synthesis of glutein and germ-like proteins, and low accumulation of protein and fat, which may result in insufficient grairt filling and low yield.